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EnduRen™ Live Cell Substrate

Live-Cell Detection of Renilla Luminescence for up to 24 Hours

  • Engineered coelenterazine for live-cell reporter gene, BRET and RNAi assays
  • Automation-friendly and highly sensitive

Size

Catalog number selected: E6481

$ 152.00 Your price: Log In

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EnduRen™ Live Cell Substrate
0.34mg
$ 152.00
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Stable Luminescence Detection for Live-Cell, High-Throughput Assays

EnduRen™ Live Cell Substrate enables live cell kinetic measurements, streamlining assay development and multiplexing with other lytic assays. EnduRen provides the ability to measure Renilla luciferase luminescence for at least 24 hours after substrate addition, with up to tenfold higher signal-to-background ratios than wildtype coelenterazines.

EnduRen™ Live Cell Substrate is a uniquely engineered coelenterazine with protected oxidation sites, which minimizes substrate degradation and autoluminescence (background) in cell culture, while it extends the luminescent signal to accommodate microplates without the need for auto-injectors. The result is that EnduRen™ Substrate overcomes the key limitations of wildtype coelenterazines by providing an automation-friendly, highly sensitive substrate for Renilla luciferase-based gene reporter and BRET applications.

Assay Advantages

  • Conserve test compounds as you create response profiles in real time to generate more data-rich results.
  • Rapidly obtain optimal assay parameters through repeat measurements using only a single cell population. Increase your sample throughput using microplates without time-consuming per-sample reagent injection steps.
  • Perform more dynamic experiments using the same sample set by pairing with any lytic assay.
  • Reliably quantitate low levels of expression for reporter gene detection and BRET.

Protocols

Specifications

You are viewing: 0.34mg Change Configuration

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Item Part # Size

EnduRen™ Live Cell Substrate

E648A 1 x 0.34mg

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,268,229 and 7,537,912 and other patents and patents pending.

Patent Pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: 3.4mg Change Configuration

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EnduRen™ Live Cell Substrate

E648B 1 x 3.4mg

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,268,229 and 7,537,912 and other patents and patents pending.

Patent Pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: 34mg Change Configuration

What's in the box?

Item Part # Size

EnduRen™ Live Cell Substrate

E648C 1 x 34mg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. Nos. 7,268,229 and 7,537,912 and other patents and patents pending.

Patent Pending.

Certain applications of this product may require licenses from others.

Resources

Citations

Bioluminescence resonance energy transfer (BRET) imaging of protein-protein interactions within deep tissues of living subjects.

--

2011 Proc. Natl. Acad. Sci. USA

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