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Dual-Luciferase® Reporter Assay System

E1910

Detect firefly and Renilla Luciferase Activities in a Single Sample

  • Renilla luciferase internal control provides more accurate results
  • Range extends over 7 logs; very active samples typically do not need dilution
  • Requires luminometer with 2 injectors

Size

Catalog number selected: E1910

$ 223.00 Your price: Log In

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Dual-Luciferase® Reporter Assay System
100 assays
$ 223.00
Your price: Log In
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Sensitive, Rapid and Convenient Dual-Reporter Assay

The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample.

The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a luminescent signal lasting at least one minute. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated simultaneously by adding Stop & Glo® Reagent to the same sample. Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors. In the DLR™ Assay System, both reporters yield linear assays with attomole (<10–18) sensitivities and no endogenous activity in the experimental host cells. Furthermore, the integrated format of the DLR™ Assay provides rapid quantitation of both reporters either in transfected cells or in cell-free transcription/translation reactions.

For best results with the Dual-Luciferase® Assay, we recommend using a luminometer that has been validated for use with the assay. These luminometers are qualified as DLReady™. For a listing of qualified instruments, please visit the DLReady™ Validated Luminometers page.

The pGL4 Luciferase Reporter Vectors are designed for use with the DLR™ Assay Systems. A Renilla luciferase vector with constitutive expression may be used in combination with any experimental firefly luciferase vector to co-transfect mammalian cells.

Notice for Cat.# E1960 and E1980: Sufficient Passive Lysis Buffer is provided to perform 1,000 assays with cells grown in 96-well plates (typically 20μl of 1X PLB per well). For applications requiring more lysis reagent (e.g., >100μl/well), additional Passive Lysis Buffer may be purchased separately.

Assay Advantages

  • Normalization to Renilla luciferase internal control allows more accurate results.
  • Samples don't have to be split, saving plates and time.
  • Allows study of weak promoters, low-level expression/regulation and expression in cells that transfect poorly.
  • Linear over 7 logs; very active samples typically do not need dilution.

Protocols

Specifications

You are viewing: E1910 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Luciferase Assay Substrate

E151A 1 × 1 vial

Passive Lysis Buffer, 5X

E194A 1 × 30ml View Product

Luciferase Assay Buffer II

E195A 1 × 10ml

Stop & Glo® Substrate

E640A 1 × 200μl

Stop & Glo® Buffer

E641A 1 × 10ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 5,744,320 and other patents.

U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents and patents pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: E1960 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Luciferase Assay Substrate

E151A 10 × 1 vial

Passive Lysis Buffer, 5X

E194A 1 × 30ml View Product

Luciferase Assay Buffer II

E195A 10 × 10ml

Stop & Glo® Substrate

E640A 10 × 200μl

Stop & Glo® Buffer

E641A 10 × 10ml

SDS

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Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 5,744,320 and other patents.

U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents and patents pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: E1980 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Stop & Glo® Reagent Bottle

E118C 1 × 1 each

Luciferase Assay Substrate

E151C 1 × 1 vial

Passive Lysis Buffer, 5X

E194A 1 × 30ml View Product

Luciferase Assay Buffer II

E195C 1 × 105ml

Stop & Glo® Substrate

E640B 2 × 1.05ml

Stop & Glo® Buffer

E641B 1 × 105ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 5,744,320 and other patents.

U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents and patents pending.

Certain applications of this product may require licenses from others.

Resources

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