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Dual-Glo® Luciferase Assay System

E2920

Detects firefly and Renilla Luciferase Activities in a Single Sample

  • Renilla luciferase internal control provides more accurate results
  • Assay cells directly in growth medium for both reporters
  • Extended half-life of 2 hours for each reporter

Size

Catalog number selected: E2920

$ 232.00 Your price: Log In

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Dual-Glo® Luciferase Assay System
10ml
$ 232.00
Your price: Log In
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An Extended-Life Dual-Luciferase Assay with "Add-Mix-Measure" Convenience

The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. The convenient "add-mix-measure" protocol generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed.

With the Dual-Glo® System, internal controls can be established to minimize sample variability by reducing false-positive and false-negative readings caused by nonspecific factors such as cytotoxicity. In the Dual-Glo® Luciferase Assay, the activity of the primary reporter is correlated with the effect of specific stimuli, and the activity of the co-transfected control reporter provides an internal control to normalize results.

The system is optimized for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media. The Dual-Glo® Luciferase Assay System provides high Z´-factors for cell-based, high-throughput screening applications.

Assay Advantages

  • Increased Precision and Accuracy. Assay normalization using an internal control co-reporter minimizes the effects of variations in cell number and health, transfection efficiency and nonspecific cellular responses.
  • No centrifugation or pre-lysis steps required. Reagents are added directly to growth medium for both reporters.
  • Stable signal allows flexibility for batch or continuous processing of 96- and 384-well plates. Each luminescent signal can be measured for up to 2 hours after reagent addition.
  • Simple, two-step assay is compatible with any luminometer. On-board injectors not required.
  • Wide Dynamic Range allows analysis of high and low reporter activity without sample dilution. Linear over at least 6 logs of enzyme concentration for each reporter.

Learn more about custom options for this product at: www.promega.com/custom/ 
Contact Promega for information on bulk purchases.

Protocols

Specifications

You are viewing: E2920 Change Configuration

What's in the box?

Item Part # Size

Dual-Glo® Luciferase Substrate

E297A 1 × 1 vial

Dual-Glo® Luciferase Buffer

E298A 1 × 10ml

Dual-Glo® Stop & Glo® Substrate

E313A 1 × 100μl

Dual-Glo® Stop & Glo® Buffer

E314A 1 × 10ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. No. 5,744,320 and other patents.

U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents and patents pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: E2940 Change Configuration

What's in the box?

Item Part # Size

Dual-Glo® Luciferase Substrate

E297B 1 × 1 vial

Dual-Glo® Luciferase Buffer

E298B 1 × 100ml

Dual-Glo® Stop & Glo® Substrate

E313B 1 × 1,000μl

Dual-Glo® Stop & Glo® Buffer

E314B 1 × 100ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. No. 5,744,320 and other patents.

U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents and patents pending.

Certain applications of this product may require licenses from others.

Specifications

You are viewing: E2980 Change Configuration

What's in the box?

Item Part # Size

Dual-Glo® Luciferase Substrate

E297B 10 × 1 vial

Dual-Glo® Luciferase Buffer

E298B 10 × 100ml

Dual-Glo® Stop & Glo® Substrate

E313B 10 × 1,000μl

Dual-Glo® Stop & Glo® Buffer

E314B 10 × 100ml

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. No. 5,744,320 and other patents.

U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents and patents pending.

Certain applications of this product may require licenses from others.

Resources

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