The Chroma-Glo™ Luciferase Assay System and the Chroma-Luc™ Vectors provide a method to generate red and green (dual-color) luminescence from a single sample upon a single-reagent addition. Filtered measurement of the dual-color luminescence produced by the Chroma-Luc™ luciferases permits each reporter to be measured independently and virtually simultaneously.
The Chroma-Glo™ Assay is in a homogeneous format that generates luminescence with >30-minute signal half-lives for each of the Chroma-Luc™ luciferases, thereby enabling the processing of many plates without prior sample handling.
The high-homology Chroma-Luc™ luciferases can be used to establish an ideal internal control for normalizing cytotoxicity in downregulation applications and for decreasing inter- and intrasample variability. You can also use the reporters to multiplex experimental reporters to increase the data content from cell-based assays.