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β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

Colorimetric Detection of β-Gal Activity

  • Safe, non-isotopic assay
  • Can be used in 96-well plate format
  • Reporter Lysis Buffer allows luciferase, CAT and β-gal assays to be performed from the same cell extract

Size

Catalog number selected: E2000

$ 167.00 Your price: Log In

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β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer
10ml
$ 167.00
Your price: Log In

Simple Detection of β-Gal Activity in Bacterial and Mammalian Cell Lysates

The β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer is a convenient method for assaying β-galactosidase activity in lysates prepared from cells transfected with β-galactosidase reporter vectors such as the pSV-β-Galactosidase Control Vector.

The standard assay is performed by adding a dilute sample to an equal volume of Assay 2X Buffer that contains the substrate ONPG (o-nitrophenyl-β-d-galactopyranoside). Samples are incubated for at least 30 minutes, during which time the β-Galactosidase hydrolyzes the colorless substrate to o-nitrophenyl, which is yellow. The reaction can be terminated by addition of sodium carbonate, and the absorbance at 420nm is measured by spectrophotometry.

Cat.# E2000 contains sufficient reagents for 65 standard assays or 200 assays in a 96-well plate format.

References
  1. Miller, J.H., ed. (1972) Experiments in Molecular Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
  2. Sambrook, J. et al. (1989) Molecular Cloning, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
  3. Schenborn, E. and Goiffon, V. (1993) Promega Notes 41, 11–3.

Protocols

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Sodium Carbonate

E202A 1 x 35ml1M

Assay 2X Buffer

E203A 1 x 10ml

Reporter Lysis 5X Buffer

E397A 1 x 30ml View Product

β-Galactosidase

E801A 1 x 100u

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

See Protocol for detailed storage recommendations.

Patents and Disclaimers

Certain applications of this product may require licenses from others.

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