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Protein analysis

Protein Labeling and Detection

Whether you are looking for secondary antibody substrates and detection systems for Western blotting or want to add a nonradioactive label to your protein during translation, we have an array of products for your protein analysis needs. Browse the HaloTag® technology products to find reagents and systems for creating fusion proteins to use with cell imaging, protein localization, purification, and protein interactions. You can find more than just the basics for labeling and detecting proteins for your proteomic investigations.

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33 Products


Applications

Accessory Reagents

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HaloTag® Vectors and Ligands Available for Sale by Quote

Pre-built HaloTag® fusion vectors and functional ligands for protein labeling and tracking.

HaloTag® Ligands for Super Resolution Microscopy

The Janelia Fluor® 549 and Janelia Fluor® 646 HaloTag® Ligands enable fluorescent labeling, confocal imaging and high-resolution single-molecule imaging studies of HaloTag® fusion proteins in living cells.

GA1110, GA1111, GA1120, GA1121

HaloTag® Fluorescent Ligands

For protein labeling and detection. Each includes a chloroalkane group for HaloTag® protein attachment.

G8251, G8252, G2801, G2802, G8272, G8273, G8581, G8582, G1001, G1002, G8471, G8472, G2991, G3221, G8281, G8282, G8591, G8592

Nano-Glo® HiBiT Blotting System

Bioluminescent method for rapidly detecting HiBiT-tagged proteins on blots without antibodies.

N2410

Transcend™ Non-Radioactive Translation Detection Systems

Non-radioactive detection of proteins synthesized in vitro.

L5070, L5080

HaloTag® Protein Purification System

Enhanced expression and solubility of recombinant proteins with HaloTag® Ligands.

G6280, G6270

pFN29 and pFC30 His6HaloTag® T7 Flexi® Vectors

Generate C- or N-terminal polyhistidine-HaloTag® fusion proteins for inducible expression in E. coli and cell-free systems.

G8261, G8331, G8321, G8381

pHTC HaloTag® CMV-neo Vector

Simple multiple cloning site (MCS) HaloTag® C-terminal vector for traditional cloning.

G7711

pHTN HaloTag® CMV-neo Vector

Configured to append HaloTag® tag to the aminoterminus of the fused protein. Vector provides constitutive high-level protein expression in mammalian cells.

G7721

pFN28A and pFN28K HaloTag® CMV-neo Flexi® Vectors

Appends HaloTag® tag to the aminoterminus of the protein fusion partner.

G8441, G8451

pFC27A and pFC27K HaloTag® CMV-neo Flexi® Vectors

HaloTag® C-terminal Flexi® Vectors for directional cloning of protein-coding sequences.

G8421, G8431

HaloTag® Mammalian Protein Purification System

Highly pure recombinant protein from mammalian cell cultures, including proteins expressed at low levels.

G6790, G6795, G6799

TMB Stabilized Substrate for Horseradish Peroxidase

Stable, ready-to-use color development substrate.

W4121

ProtoBlot® II AP Systems with Stabilized Substrate and Western Express™ Fast Blotting Protocol

Rapid, sensitive detection of proteins immobilized on nitrocellulose or PVDF membranes.

W3940, W3950, W3960

Blocking Agents

Bovine serum albumin is used in immunoblotting applications with AP antibody conjugates. Tween® 20 dissociates nonspecific interactions with an antibody probe.

W3841, W3831

Showing 33 of 33 Products

Why Label and Detect Proteins?

Labeling and detecting proteins are integral to protein analysis. By incorporating an amino acid with a colorimetric or fluorescent moiety during in vitro translation, you can label a protein for further study. For example, adding a nonradioactive label when using a cell-free expression system means you can detect your labeled protein on a gel rather than a membrane. You can also label proteins using a peptide tag. Once you have fused your protein with a tag at the C or N terminus, you can further analyze protein function. Not only can you purify your tagged protein, you can also use the tag for antibody-based detection (e.g., Western blotting), pull-down assays and more. Determining how well your protein was expressed during translation or where your protein resides in the cell is all information that can help you understand what role the protein plays. Labeling a protein is only the first step. Detection systems help you figure out where it is, how much there is, and what its role might be in the cell.

 

We offer a complete portfolio of protein analysis products supported by a growing library of educational resources, including technology guides, on-demand webinars, scientific articles and protocols.

Use the links below to learn more about protein analysis products, or to explore learning resources covering topics such as in vitro protein expression, protein purification methods and live-cell protein interaction analysis.