The PinPoint™ Vector Sequencing Primer is designed for sequencing inserts cloned into the PinPoint™ Xa Vectors (components of Cat.# V2020). The primer hybridizes upstream of the Factor Xa site at nucleotides 325–343, approximately 40–50 base pairs upstream of the multiple cloning region, and can be used to determine if an insert is cloned in-frame with the biotinylation purification tag of the PinPoint™ Xa Vectors. The sequence of the PinPoint™ Vector Sequencing Primer is 5´-d(CGTGACGCGGTGCAGGGCG)-3´. It is supplied dried.
Performance Tested: The PinPoint™ Vector Sequencing Primer is tested in double-stranded sequencing reactions with circular PinPoint™ Vectors.