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Maxwell® 16 Polyhistidine Protein Purification Kit

Automated Purification of His-Tagged Protein from Bacterial, Insect and Mammalian Cell Cultures

  • Use with culture types including bacterial, mammalian and insect cells and culture medium
  • Prefilled cartridges eliminate reagent preparation and multiple pipetting steps, centrifugation, additional sample manipulation

Size

Catalog number selected: AS1060

$ 373.00 Your price: Log In

Maxwell® 16 Polyhistidine Protein Purification Kit
48 preps
$ 373.00
Your price: Log In

Automated His-Tagged Protein Purification from Bacterial, Mammalian or Insect Cells

The Maxwell® 16 Polyhistidine Protein Purification Kit is used with the Maxwell® 16 Instrument to provide an easy method for the efficient, automated purification of polyhistidine-tagged protein from bacterial cultures and other sample types including mammalian and insect cells. With minor modifications, the reagents can also be used for purification of HQ-tagged proteins from bacterial cultures.

The Maxwell® Instrument is supplied with a preprogrammed purification procedure and reagent cartridges specifically designed to maximize simplicity and convenience. The instrument can process up to 16 samples in approximately 40 minutes. The purified protein is compatible with downstream applications such as gel electrophoresis and Western blot analysis.

Applications

  • Polyhistidine-tagged protein purification
  • HQ-tagged protein purification
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Intra-run and inter-run consistency of luciferase purification using the Maxwell® 16 Polyhistidine Protein Purification Kit and the Maxwell® 16 Instrument.

Maxwell® Cartridge Seal Change

The seal length on Maxwell® Cartridges has changed to create a pull-tab feature. The manufacturing procedures and quality-control testing of products have not changed.

If you have any questions, please contact us: 
In North America, call Promega Technical Services at 800-356-9526.
Outside North America, contact your branch office or call Promega Technical Services at 608-274-4330.
Worldwide, e-mail Promega Technical Services at: techserv@promega.com

Protocols

Specifications

What's in the box?

Item Part # SizeAvailable Separately

Elution Tubes

AS510A 1 x 50/pk View Product

Plungers

AS520A 1 x 50/pk View Product

Maxwell® 16 Protein Cartridge A

AS570A 48 x 1 each

Elution Buffer

V852E 1 x 16ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Patents and Disclaimers

U.S. Pat. Nos. 7,112,552 and 7,354,750.

This product is licensed for use under U.S. Pat. No. 6,174,704.

Resources

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Small Molecule Drug Discovery Workflow

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