MagZ™ Protein Purification System provides a simple, rapid and reliable method for the purification of expressed polyhistidine- or HQ-tagged proteins, which are 99% free of hemoglobin contamination, from rabbit reticulocyte lysate. Based on the use of proprietary, paramagnetic precharged particles, polyhistidine- or HQ-tagged protein can be isolated from 50–500μl of TnT® Coupled Transcription/Translation reactions. Polyhistidine- or HQ-tagged proteins bind to the particles in minutes, while unbound proteins are washed away, and the target protein is eluted with imidazole.
Benefits include minimal hemoglobin (<0.1%) binding to the MagZ™ Binding Particles, no long incubations required, and the ability to further optimize binding/wash and elution conditions for individual polyhistidine- or HQ-tagged proteins.
Note: This product requires the use of a magnetic stand.
For more information, see the Protocols & Applications Guide.
- Expression and purification of polyhistidine-tagged proteins in rabbit reticulocyte lysate with less than 1% hemoglobin contamination.