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MagZ™ Protein Purification System

MagZ™ Protein Purification System provides a simple, rapid and reliable method for the purification of expressed polyhistidine- or HQ-tagged proteins, which are 99% free of hemoglobin contamination, from rabbit reticulocyte lysate. Based on the use of proprietary, paramagnetic precharged particles, polyhistidine- or HQ-tagged protein can be isolated from 50–500μl of TnT® Coupled Transcription/Translation reactions. Polyhistidine- or HQ-tagged proteins bind to the particles in minutes, while unbound proteins are washed away, and the target protein is eluted with imidazole.

Benefits include minimal hemoglobin (<0.1%) binding to the MagZ™ Binding Particles, no long incubations required, and the ability to further optimize binding/wash and elution conditions for individual polyhistidine- or HQ-tagged proteins.

Note: This product requires the use of a magnetic stand.

For more information, see the Protocols & Applications Guide.

Application

  • Expression and purification of polyhistidine-tagged proteins in rabbit reticulocyte lysate with less than 1% hemoglobin contamination.

Overview of the MagZ™ Protein Purification System protocol.

Overview of the MagZ™ Protein Purification System protocol.
Overview of the MagZ™ Protein Purification System protocol.

Specifications

What's in the box?

Item Part # Size

MagZ™ Binding Particles

V884A 1 × 2ml

MagZ™ Binding/Wash Buffer

V885A 1 × 40ml

MagZ™ Elution Buffer

V886A 1 × 20ml

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

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