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HisLink™ Protein Purification Systems

Capture His- or HQ-Tagged Proteins Cell Lysates by Centrifugation or 96-Well Plate

  • Tagged proteins purified directly from cell lysates
  • Purify manually by batch, using a vacuum manifold, liquid chromatography or liquid handling platform
  • Purify by centrifugation or vacuum method

Choose a HisLink™ System

Size

Catalog number selected: V3680

$ 341.00 Your price: Log In

HisLink™ Protein Purification Systems
HisLink™ 96/1 × 96
$ 341.00
Your price: Log In
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Protein Purification from Bacterial Cultures; You Choose the Method

HisLink™ Protein Purification Systems are designed for purification of polyhistidine (His)-tagged or HQ-tagged proteins directly from culture medium containing bacterial cells expressing the tagged protein of interest. Both the "spin" and "96" formats use the HisLink™ Protein Purification Resin (Cat.# V8821), a macroporous silica resin derivatized with a high level of tetradentate-chelated nickel (>20mmol Ni/ml settled resin).

The resin performs well in either column, batch or vacuum-based methods with a binding capacity of >15mg/ml of resin. HisLink™ Protein Purification Resin is useful in all general immobilized metal affinity chromatography (IMAC) applications matrix as well as in low- to medium-pressure liquid chromatography systems.

The bacterial cells are lysed using the FastBreak™ Cell Lysis Reagent (Cat.# V8573), and the crude lysate is combined with the HisLink™ Resin. The addition of these reagents results in simultaneous bacterial lysis and binding of the polyhistidine- or HQ-tagged proteins. No long lysozyme incubations are required for cell lysis.

The resin can be used with vacuum filtration devices (e.g., Vac-Man® Vacuum Manifold [Cat.# A7231]) to rapidly process simultaneous samples. The HisLink™ 96 Protein Purification System (Cat.# V3680) provides a simple and quick vacuum-based method of simultaneously purifying multiple His-tagged proteins  from E. coli cultured in deep-well, 96-well plates. The HisLink™ 96 System is amenable to manual or automated methods, such as the Beckman Coulter Biomek® 2000 or FX for high-throughput applications, yielding 1mg of purified polyhistidine-tagged protein per well.

Note: Resin product volumes represent total volume of a 50% resin slurry.

Applications

  • Protein purification of polyhistidine- or HQ-tagged proteins using IMAC.
  • Purification of tagged proteins from E. coli using a vacuum- or centrifugation-based format.
  • Protein purification of polyhistidine- or HQ-tagged proteins from 96-well, deep-well culture plates using IMAC.

For more information, see the Protocols & Applications Guide.

5583MA.eps
Overview of polyhistidine- or HQ-tagged protein purification using the HisLink™ Spin Protein Purification System.

Protocols

Specifications

You are viewing: 1 × 96 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

HisLink™ 96 Filtration/Collection Plates

V484A 1 x 1 pack

Binding/Wash Buffer

V851C 1 x 110ml

Elution Buffer

V852C 1 x 25ml

FastBreak™ Cell Lysis Reagent, 10X

V857A 1 x 15ml View Product

HisLink™ Protein Purification Resin

V882B 1 x 25ml View Product

DNase I (lyophilized)

Z358B 1 x 1 vial

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,112,552 and 7,354,750.

This product is licensed for use under U.S. Pat. No. 6,174,704.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Specifications

You are viewing: 5 × 96 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

HisLink™ 96 Filtration/Collection Plates

V484A 5 x 1 pack

Binding/Wash Buffer

V851D 2 x 280ml

Elution Buffer

V852D 1 x 125ml

FastBreak™ Cell Lysis Reagent, 10X

V857B 1 x 60ml View Product

HisLink™ Protein Purification Resin

V882A 2 x 50ml View Product

DNase I (lyophilized)

Z358A 1 x 1 vial

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,112,552 and 7,354,750.

This product is licensed for use under U.S. Pat. No. 6,174,704.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

Specifications

You are viewing: 25 reactions Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Collection Tubes

A130D 1 x 25/pk

FastBreak™ Cell Lysis Reagent, 10X

V857D 1 x 5ml View Product

HisLink™ Protein Purification Resin

V882C 1 x 5ml View Product

HisLink™ Binding/Wash Buffer

V981A 1 x 50ml

HisLink™ Elution Buffer

V982A 1 x 10ml

Spin Column

V995A 1 x 25 each

DNase I (lyophilized)

Z358B 1 x 1 vial

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,112,552 and 7,354,750.

This product is licensed for use under U.S. Pat. No. 6,174,704.

Certain applications of this product may be covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

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