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HisLink™ Protein Purification Systems

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HisLink™ Protein Purification Systems

Protein Purification from Bacterial Cultures; You Choose the Method

HisLink™ Protein Purification Systems are designed for purification of polyhistidine (His)-tagged or HQ-tagged proteins directly from culture medium containing bacterial cells expressing the tagged protein of interest. using the HisLink™ Protein Purification Resin (Cat.# V8821), a macroporous silica resin derivatized with a high level of tetradentate-chelated nickel (>20mmol Ni/ml settled resin).

The resin performs well in either column, batch or vacuum-based methods with a binding capacity of >15mg/ml of resin. HisLink™ Protein Purification Resin is useful in all general immobilized metal affinity chromatography (IMAC) applications matrix as well as in low- to medium-pressure liquid chromatography systems.

The bacterial cells are lysed using the FastBreak™ Cell Lysis Reagent (Cat.# V8573), and the crude lysate is combined with the HisLink™ Resin. The addition of these reagents results in simultaneous bacterial lysis and binding of the polyhistidine- or HQ-tagged proteins. No long lysozyme incubations are required for cell lysis.

The resin can be used with vacuum filtration devices (e.g., Vac-Man® Vacuum Manifold [Cat.# A7231]) to rapidly process simultaneous samples.

Note: Resin product volumes represent total volume of a 50% resin slurry.

Applications

  • Protein purification of polyhistidine- or HQ-tagged proteins using IMAC.
  • Purification of tagged proteins from E. coli using a vacuum- or centrifugation-based format.

For more information, see the Protocols & Applications Guide.

Specifications

What's in the box?

Item Part # Size Available Separately

Collection Tubes

A130D 1 × 25/pk

FastBreak™ Cell Lysis Reagent, 10X

V857D 1 × 5ml View Product

HisLink™ Protein Purification Resin

V882C 1 × 5ml View Product

HisLink™ Binding/Wash Buffer

V981A 1 × 50ml

HisLink™ Elution Buffer

V982A 1 × 10ml

Spin Column

V995A 1 × 25 each

DNase I (lyophilized)

Z358B 1 × 1 vial

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

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Jan

Jan

Germany