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HisLink™ Protein Purification Systems

Capture His- or HQ-Tagged Protein Cell Lysates by Centrifugation or 96-Well Plate

  • His tagged or HQ tagged proteins purified directly from cell lysates
  • Purify manually by batch, using a vacuum manifold, liquid chromatography or liquid handling platform
  • Purify by centrifugation or vacuum method

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Catalog number selected: V1320

$ 250.00
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HisLink™ Protein Purification Systems
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Protein Purification from Bacterial Cultures; You Choose the Method

HisLink™ Protein Purification Systems are designed for purification of polyhistidine (His)-tagged or HQ-tagged proteins directly from culture medium containing bacterial cells expressing the tagged protein of interest. using the HisLink™ Protein Purification Resin (Cat.# V8821), a macroporous silica resin derivatized with a high level of tetradentate-chelated nickel (>20mmol Ni/ml settled resin).

The resin performs well in either column, batch or vacuum-based methods with a binding capacity of >15mg/ml of resin. HisLink™ Protein Purification Resin is useful in all general immobilized metal affinity chromatography (IMAC) applications matrix as well as in low- to medium-pressure liquid chromatography systems.

The bacterial cells are lysed using the FastBreak™ Cell Lysis Reagent (Cat.# V8573), and the crude lysate is combined with the HisLink™ Resin. The addition of these reagents results in simultaneous bacterial lysis and binding of the polyhistidine- or HQ-tagged proteins. No long lysozyme incubations are required for cell lysis.

The resin can be used with vacuum filtration devices (e.g., Vac-Man® Vacuum Manifold [Cat.# A7231]) to rapidly process simultaneous samples.

Note: Resin product volumes represent total volume of a 50% resin slurry.

Applications

  • Protein purification of polyhistidine- or HQ-tagged proteins using IMAC.
  • Purification of tagged proteins from E. coli using a vacuum- or centrifugation-based format.

For more information, see the Protocols & Applications Guide.

Overview of polyhistidine- or HQ-tagged protein purification using the HisLink™ Spin Protein Purification System.

Overview of polyhistidine- or HQ-tagged protein purification using the HisLink™ Spin Protein Purification System.
Overview of polyhistidine- or HQ-tagged protein purification using the HisLink™ Spin Protein Purification System.

Specifications

What's in the box?

Item Part # SizeAvailable Separately

Collection Tubes

A130D 1 × 25/pk

FastBreak™ Cell Lysis Reagent, 10X

V857D 1 × 5ml View Product

HisLink™ Protein Purification Resin

V882C 1 × 5ml View Product

HisLink™ Binding/Wash Buffer

V981A 1 × 50ml

HisLink™ Elution Buffer

V982A 1 × 10ml

Spin Column

V995A 1 × 25 each

DNase I (lyophilized)

Z358B 1 × 1 vial

SDS

Choose language:

Download SDSPDF (202 KB) – English (United States)

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

See Protocol for detailed storage recommendations.

Patents and Disclaimers

U.S. Pat. Nos. 7,112,552 and 7,354,750.

This product is licensed for use under U.S. Pat. No. 6,174,704.

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