N200 FRB-LgBiT Control Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 N200 FRB-LgBiT Control Vector Vector sequence reference points: Base pairs 4762 HSV-TK promoter 1-753 FRB-LgBiT 802-1614 Sv40 late polyadenylation signal 1751-1972 SV40 enhancer and early promoter 2071-2489 SV40 Enhancer 2144-2380 (Reverse) Sv40 Min Ori 2387-2452 EM7 bacterial promoter 2497-2563 Neo-Kan Resistance 2577-3371 Synthetic PolyAdenylation Signal Sequence 3435-3483 ColE1-derived plasmid replication origin 3719-3755 ---- 1 AAATGAGTCT TCGGACCTCG CGGGGGCCGC TTAAGCGGTG GTTAGGGTTT 51 GTCTGACGCG GGGGGAGGGG GAAGGAACGA AACACTCTCA TTCGGAGGCG 101 GCTCGGGGTT TGGTCTTGGT GGCCACGGGC ACGCAGAAGA GCGCCGCGAT 151 CCTCTTAAGC ACCCCCCCGC CCTCCGTGGA GGCGGGGGTT TGGTCGGCGG 201 GTGGTAACTG GCGGGCCGCT GACTCGGGCG GGTCGCGCGC CCCAGAGTGT 251 GACCTTTTCG GTCTGCTCGC AGACCCCCGG GCGGCGCCGC CGCGGCGGCG 301 ACGGGCTCGC TGGGTCCTAG GCTCCATGGG GACCGTATAC GTGGACAGGC 351 TCTGGAGCAT CCGCACGACT GCGGTGATAT TACCGGAGAC CTTCTGCGGG 401 ACGAGCCGGG TCACGCGGCT GACGCGGAGC GTCCGTTGGG CGACAAACAC 451 CAGGACGGGG CACAGGTACA CTATCTTGTC ACCCGGAGGC GCGAGGGACT 501 GCAGGAGCTT CAGGGAGTGG CGCAGCTGCT TCATCCCCGT GGCCCGTTGC 551 TCGCGTTTGC TGGCGGTGTC CCCGGAAGAA ATATATTTGC ATGTCTTTAG 601 TTCTATGATG ACACAAACCC CGCCCAGCGT CTTGTCATTG GCGAAGTCGA 651 ACACGCAGAT GCAGTCGGGG CGGCGCGGTC CCAGGTCCAC TTCGCATATT 701 AAGGTGACGC GTGTGGCCTC GAACACCGAG CGACCCTGCA GCGACCCGCT 751 TAAAAGCTTG GCAATCCGGT ACTGTTGGTA AAGCCACCGC TAGCGATCGC 801 CATGGTGGCC ATCCTCTGGC ATGAGATGTG GCATGAAGGC CTGGAAGAGG 851 CATCTCGTTT GTACTTTGGG GAAAGGAACG TGAAAGGCAT GTTTGAGGTG 901 CTGGAGCCCT TGCATGCTAT GATGGAACGG GGCCCCCAGA CTCTGAAGGA 951 AACATCCTTT AATCAGGCCT ATGGTCGAGA TTTAATGGAG GCCCAAGAGT 1001 GGTGCAGGAA GTACATGAAA TCAGGGAATG TCAAGGACCT CACCCAAGCC 1051 TGGGACCTCT ATTATCATGT GTTCCGACGA ATCTCAGTTT CTCAAGGCAG 1101 TTCAGGTGGT GGCGGGAGCG GAGGTGGAGG CTCGAGCGGT GTCTTCACAC 1151 TCGAAGATTT CGTTGGGGAC TGGGAACAGA CAGCCGCCTA CAACCTGGAC 1201 CAAGTCCTTG AACAGGGAGG TGTGTCCAGT TTGCTGCAGA ATCTCGCCGT 1251 GTCCGTAACT CCGATCCAAA GGATTGTCCG GAGCGGTGAA AATGCCCTGA 1301 AGATCGACAT CCATGTCATC ATCCCGTATG AAGGTCTGAG CGCCGACCAA 1351 ATGGCCCAGA TCGAAGAGGT GTTTAAGGTG GTGTACCCTG TGGATGATCA 1401 TCACTTTAAG GTGATCCTGC CCTATGGCAC ACTGGTAATC GACGGGGTTA 1451 CGCCGAACAT GCTGAACTAT TTCGGACGGC CGTATGAAGG CATCGCCGTG 1501 TTCGACGGCA AAAAGATCAC TGTAACAGGG ACCCTGTGGA ACGGCAACAA 1551 AATTATCGAC GAGCGCCTGA TCACCCCCGA CGGCTCCATG CTGTTCCGAG 1601 TAACCATCAA CAGCTAAGGC CGCGACTCTA GAGTCGACCT GCAGGCATGC 1651 AAGCTGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT 1701 GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCGG CCGCTTCGAG 1751 CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG 1801 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT 1851 TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC 1901 ATTTTATGTT TCAGGTTCAG GGGGAGATGT GGGAGGTTTT TTTAAGCAAG 1951 TAAAACCTCT ACAAATGTGG TAAAATCGAA TTTTAACAAA ATATTAACGC 2001 TTACAATTTC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT 2051 CACACCGCAT ACGCGGATCT GCGCAGCACC ATGGCCTGAA ATAACCTCTG 2101 AAAGAGGAAC TTGGTTAGGT ACCTTCTGAG GCGGAAAGAA CCAGCTGTGG 2151 AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG 2201 AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT 2251 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 2301 TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC 2351 GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT 2401 ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA 2451 GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTA ATTAACTGTT 2501 GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT 2551 GAGGAACTAA ACCCAGGAGG CAGATCATGA TTGAACAAGA TGGATTGCAC 2601 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC 2651 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC 2701 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 2751 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT 2801 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC 2851 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 2901 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC 2951 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG 3001 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 3051 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA 3101 GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT 3151 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 3201 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC 3251 CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG 3301 TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 3351 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC 3401 CAAGCGACGC CCAACCTGCC ATCACGATGG CCGCAATAAA ATATCTTTAT 3451 TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGAATCGAT AGCGATAAGG 3501 ATCCTCTTTG CGCTTGCGTT TTCCCTTGTC CAGATAGCCC AGTAGCTGAC 3551 ATTCATCCGG GGTCAGCACC GTTTCTGCGG ACTGGCTTTC TACCCGGTAT 3601 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC 3651 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 3701 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 3751 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 3801 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 3851 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 3901 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG 3951 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 4001 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 4051 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 4101 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 4151 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 4201 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT 4251 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 4301 AGGATTTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 4351 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 4401 ATCTTCACCT AGATCCTTTT ATAGTCCGGA AATACAGGAA CGCACGCTGG 4451 ATGGCCCTTC GCTGGGATGG TGAAACCATG AAAAATGGCA GCTTCAGTGG 4501 ATTAAGTGGG GGTAATGTGG CCTGTACCCT CTGGTTGCAT AGGTATTCAT 4551 ACGGTTAAAA TTTATCAGGC GCGATTGCGG CAGTTTTTCG GGTGGTTTGT 4601 TGCCATTTTT ACCTGTCTGC TGCCGTGATC GCGCTGAACG CGTTTTAGCG 4651 GTGCGTACAA TTAAGGGATT ATGGTAAATC CACTTACTGT CTGCCCTCGT 4701 AGCCATCGAG ATAAACCGCA GTACTCCGGC CACGATGCGT CCGGCGTAGA 4751 GGATCGAGAT CT Sequence file prepared 11/20/2015 by Feature database and feature finder version 1.0.4.2.