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ProtoBlot® II AP Systems with Stabilized Substrate and Western Express™ Fast Blotting Protocol

Rapid, Sensitive Alkaline Phosphatase Detection of Proteins Immobilized on Nitrocellulose or PVDF Membranes

  • Western Express™ Protocol allows dot blot detection in 30–45 minutes, Western blot detection in 1–2 hours
  • Tenfold greater detection sensitivity than HRP (horseradish peroxidase)
  • Choose system compatible with human, mouse or rabbit

Choose a Conjugate

Size

Catalog number selected: W3940

$ 247.00 Your price: Log In

ProtoBlot® II AP Systems with Stabilized Substrate and Western Express™ Fast Blotting Protocol
Anti-Human IgG AP Conjugate/1 system
$ 247.00
Your price: Log In
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Designed for rapid, sensitive alkaline phosphatase (AP) detection of proteins or other macromolecular antigens immobilized on nitrocellulose or PVDF membranes. Proteins can be transferred from gels after electrophoresis (Western blots) or bound directly from solution ("dot" blots). The Western Express™ Fast Blotting Protocol is included with the system and can reduce dramatically the time required to perform immunodetection, with detection of dot blots in 30–45 minutes and Western blots in 1–2 hours.

The ProtoBlot® II AP Systems contain BSA as a stabilizer and 0.05% sodium azide as a preservative. In addition, the system contains Western Blue® Stabilized Substrate for AP, a ready-to-use solution of BCIP/NBT.

For many applications, AP conjugates are superior to HRP conjugates:

  • Tenfold greater sensitivity of detection
  • Not inhibited by the presence of azide
  • Substrate is not prone to fading during long-term storage
  • Protocols provided for both PVDF and nitrocellulose membranes

References

  1. Towbin, H. et al. (1979) Proc. Natl. Acad. Sci. USA 76, 4350–4.
  2. Burnette, W.N. (1981) Anal. Biochem. 112, 195–203.
  3. Hawkes, R. et al. (1982) Anal. Biochem. 119, 142–7.
  4. Larson, G. (1992) Promega Notes 35, 18–20.
  5. Blake, M.S. et al. (1984) Anal. Biochem. 136, 175–9.

Protocols

Specifications

You are viewing: 1 each Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Anti-Human IgG (H+L) AP Conjugate

S382B 1 x 100μl View Product

Western Blue® Stabilized Substrate for Alkaline Phosphatase

S3841 3 x 100ml

Tween® 20

W383B 1 x 5ml View Product

Blot-Qualified BSA

W384A 1 x 10g View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

 

Specifications

You are viewing: 1 each Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Anti-Mouse IgG (H+L) AP Conjugate

S372B 1 x 100μl View Product

Western Blue® Stabilized Substrate for Alkaline Phosphatase

S3841 3 x 100ml

Tween® 20

W383B 1 x 5ml View Product

Blot-Qualified BSA

W384A 1 x 10g View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

 

Specifications

You are viewing: 1 each Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Anti-Rabbit IgG (Fc) AP Conjugate

S373B 1 x 100μl View Product

Western Blue® Stabilized Substrate for Alkaline Phosphatase

S3841 3 x 100ml

Tween® 20

W383B 1 x 5ml View Product

Blot-Qualified BSA

W384A 1 x 10g View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

See Protocol for detailed storage recommendations.

 

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