pFC14A (HaloTag(R) 7) CMV Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC14A (HaloTag(R) 7) CMV Flexi(R) Vector sequence reference points: Base pairs 5056 CMV promoter 1-742 Chimeric intron 857-989 T7 RNA polymerase promoter (-17 to +3) 1033-1052 SgfI region 1056-1063 barnase coding region 1087-1422 HT7 linker C terminal 1447-1491 TEV protease recognition sequence 1462-1482 HT7 tag C terminal 1492-2382 SV40 late poly(A) signal 2516-2737 beta-lactamase (Ampr) coding region 2998-3858 ColE1-derived plasmid origin of replication 4013-4049 Cer region (site for E. coli XerCD recombinase) 4720-5005 .. 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGAT CGCCATGGAA TAAGTAAGGA ATCCACATGG CACAGGTTAT 1101 CAACACGTTT GACGGGGTTG CGGATTATCT TCAGACATAT CATAAGCTAC 1151 CTGATAATTA CATTACAAAA TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA 1201 TCAAAAGGGA ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA 1251 CATCTTCTCA AACAGGGAAG GCAAACTCCC GGGCAAAAGC GGACGAACAT 1301 GGCGTGAAGC GGATATTAAC TATACATCAG GCTTCAGAAA TTCAGACCGG 1351 ATTCTTTACT CAAGCGACTG GCTGATTTAC AAAACAACGG ACCATTATCA 1401 GACCTTTACA AAAATCAGAT AATGTTTAAT GACCCCGTGT CGAGCTCTCG 1451 AGCCAACCAC TGAGGATCTG TACTTTCAGA GCGATAACGA TGGATCCGAA 1501 ATCGGTACTG GCTTTCCATT CGACCCCCAT TATGTGGAAG TCCTGGGCGA 1551 GCGCATGCAC TACGTCGATG TTGGTCCGCG CGATGGCACC CCTGTGCTGT 1601 TCCTGCACGG TAACCCGACC TCCTCCTACG TGTGGCGCAA CATCATCCCG 1651 CATGTTGCAC CGACCCATCG CTGCATTGCT CCAGACCTGA TCGGTATGGG 1701 CAAATCCGAC AAACCAGACC TGGGTTATTT CTTCGACGAC CACGTCCGCT 1751 TCATGGATGC CTTCATCGAA GCCCTGGGTC TGGAAGAGGT CGTCCTGGTC 1801 ATTCACGACT GGGGCTCCGC TCTGGGTTTC CACTGGGCCA AGCGCAATCC 1851 AGAGCGCGTC AAAGGTATTG CATTTATGGA GTTCATCCGC CCTATCCCGA 1901 CCTGGGACGA ATGGCCAGAA TTTGCCCGCG AGACCTTCCA GGCCTTCCGC 1951 ACCACCGACG TCGGCCGCAA GCTGATCATC GATCAGAACG TTTTTATCGA 2001 GGGTACGCTG CCGATGGGTG TCGTCCGCCC GCTGACTGAA GTCGAGATGG 2051 ACCATTACCG CGAGCCGTTC CTGAATCCTG TTGACCGCGA GCCACTGTGG 2101 CGCTTCCCAA ACGAGCTGCC AATCGCCGGT GAGCCAGCGA ACATCGTCGC 2151 GCTGGTCGAA GAATACATGG ACTGGCTGCA CCAGTCCCCT GTCCCGAAGC 2201 TGCTGTTCTG GGGCACCCCA GGCGTTCTGA TCCCACCGGC CGAAGCCGCT 2251 CGCCTGGCCA AAAGCCTGCC TAACTGCAAG GCTGTGGACA TCGGCCCGGG 2301 TCTGAATCTG CTGCAAGAAG ACAACCCGGA CCTGATCGGC AGCGAGATCG 2351 CGCGCTGGCT GTCTACTCTG GAGATTTCCG GTTAATAGAA TTCTAGAGTC 2401 GACCTGCAGG CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA 2451 GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG 2501 GGCGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA 2551 ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA 2601 TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 2651 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG 2701 GTTTTTTTAA GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTCTA 2751 ATGGATCCTC TTTGCGCTTG CGTTTTCCCT TGTCCAGATA GCCCAGTAGC 2801 TGACATTCAT CCGGGGTCAG CACCGTTTCT GCGGACTGGC TTTCTACGTA 2851 ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC 2901 CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA 2951 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG 3001 AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG 3051 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG 3101 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC 3151 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 3201 CACTTTCAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG 3251 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT 3301 GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG 3351 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT 3401 TACTTCTGAC AACTATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC 3451 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 3501 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG 3551 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC 3601 CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT 3651 TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG 3701 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT 3751 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 3801 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC 3851 ATTGGTAATT CGAAATGACC GACCAAGCGA CGCCCAACCG GTATCAGCTC 3901 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA 3951 AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 4001 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 4051 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA 4101 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 4151 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 4201 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 4251 CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 4301 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 4351 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT 4401 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 4451 TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 4501 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 4551 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATT 4601 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 4651 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 4701 ACCTAGATCC TTTTATAGTC CGGAAATACA GGAACGCACG CTGGATGGCC 4751 CTTCGCTGGG ATGGTGAAAC CATGAAAAAT GGCAGCTTCA GTGGATTAAG 4801 TGGGGGTAAT GTGGCCTGTA CCCTCTGGTT GCATAGGTAT TCATACGGTT 4851 AAAATTTATC AGGCGCGATT GCGGCAGTTT TTCGGGTGGT TTGTTGCCAT 4901 TTTTACCTGT CTGCTGCCGT GATCGCGCTG AACGCGTTTT AGCGGTGCGT 4951 ACAATTAAGG GATTATGGTA AATCCACTTA CTGTCTGCCC TCGTAGCCAT 5001 CGAGATAAAC CGCAGTACTC CGGCCACGAT GCGTCCGGCG TAGAGGATCG 5051 AGATCT Vector sequence updated 24-Aug-07.