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HaloTag® Ligands for Super Resolution Microscopy

Single-Use Ligands for High-Resolution lmaging in Live Cells

  • Single-molecule labeling
  • Rapid cell labeling
  • High signal-to-noise ratio and specificity

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Catalog number selected: GA1110

$ 350.00
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This product is discontinued
HaloTag® Ligands for Super Resolution Microscopy
Janelia Fluor® 549 HaloTag®/5µg
$ 350.00
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Brighter Ligands, No-Wash Protocol

The Janelia Fluor® 549 HaloTag® Ligand and Janelia Fluor® 646 HaloTag® Ligand enable characterization of HaloTag® fusions in endogenous cellular settings. The enhanced brightness of the dyes associated with the HaloTag® ligands enables their use in detection and single-molecule imaging studies in live cells via: 

  • FACS
  • Standard confocal imaging
  • In-gel detection with a fluoroimager

Labeling of Live Cells Expressing Nuclear HaloTag® Protein

Live cell labeling with Janelia Fluor 549 HaloTag Ligand 15240ta-wa
Live cell labeling with Janelia Fluor 646 HaloTag Ligand 15240ta-wb
Parental U2OS cells and U2OS cells expressing HaloTag® containing a nuclear localization sequence were adhered to glass-bottom chamber slides and labeled with 200nM Janelia Fluor® 549 HaloTag® Ligand or Janelia Fluor® 646 HaloTag® Ligand for 15 minutes. Cells were imaged with 561nm laser excitation for Janelia Fluor® 549 HaloTag® Ligand (Panel A) and 637nm laser excitation for Janelia Fluor® 646 HaloTag® Ligand (Panel B). In HaloTag-expressing cells, labeling is restricted to the nucleus. Parental cells (live control in figure) show no labeling. The top row of each panel is fluorescent signal only. The bottom row shows transmitted images collected at the same respective laser excitation with the fluorescence images overlaid. Images were collected using a Nikon Eclipse Ti confocal microscope equipped with NIS Elements software and a 40X plan fluor oil immersion objective.

FACS of Janelia Fluor® HaloTag® Labeled Proteins

Excitation-emission spectra for Janelia Fluor 549 and 646 HaloTag Ligands  15262ta-w
HEK293 cells were edited using CRISPR/Cas9 to tag endogenous BRD4 protein with HaloTag® at the N-terminus. The edited cells pool was labeled with Janelia Fluor® 646 HaloTag® Ligand and sorted on a BD FACSMelody™ sorter to isolate single clones. After expansion of cell populations, HaloTag®-expressing clones and parental HEK293 cells labeled with Janelia Fluor® 646 HaloTag® Ligand were analyzed by flow cytometry on a BD LSRFortessa™. Clonal populations expressing HaloTag fused to endogenous BRD4 (blue histogram) showed a 5-fold shift in mean fluorescence compared to labeled parental HEK293 cells (pink histogram).

Janelia Fluor® HaloTag® Ligand References

  1. Grimm, J. et al. (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nature Methods 12, 244–50.
  2. Courtney, N. et al. (2018) Excitatory and inhibitory neurons utilize different Ca2+ sensors and sources to regulate spontaneous release. Neuron 98, 977–91.
  3. Presman, D.M. et al. (2017) Quantifying transcription factor binding dynamics at the single-molecule level in live cells. Methods 123, 76–88.
  4. Guo, M. et al. (2018) Single-shot super-resolution total internal reflection fluorescence microscopy. Nature Methods 15, 425–8.
  5. Chong, S. et al. (2018) Imaging dynamic and selective low-complexity domain interactions that control gene transcription. Science DOI: 10.1126/science.aar2555

Specifications

You are viewing: GA1110 Change Configuration

What's in the box?

Item Part # Size

Janelia Fluor® 549 HaloTag® Ligand

GA111A 1 × 5μg

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

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Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.

Licensed under patents pending from Howard Hughes Medical Institute.

Specifications

You are viewing: GA1111 Change Configuration

What's in the box?

Item Part # Size

Janelia Fluor® 549 HaloTag® Ligand

GA111A 3 × 5μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.

Licensed under patents pending from Howard Hughes Medical Institute.

Specifications

You are viewing: GA1120 Change Configuration

What's in the box?

Item Part # Size

Janelia Fluor® 646 HaloTag® Ligand

GA112A 1 × 5μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.

Licensed under patents pending from Howard Hughes Medical Institute.

Specifications

You are viewing: GA1121 Change Configuration

What's in the box?

Item Part # Size

Janelia Fluor® 646 HaloTag® Ligand

GA112A 3 × 5μg

SDS

Choose language:

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 7,867,726, 8,168,405 and 8,257,939 and other patents and patents pending.

Licensed under patents pending from Howard Hughes Medical Institute.