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Wheat Germ Extract

Components for Protein Synthesis

  • Contains tRNA, ribosomes, initiation, elongation and termination factors
  • Extract is cleared of endogenous amino acids, mRNA and plant pigments, minimizing background translation

Size

Catalog number selected: L4380

$ 197.00 Your price: Log In

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Wheat Germ Extract
5 × 200μl
$ 197.00
Your price: Log In

Contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors). Wheat Germ Extract preparation include grinding in an extraction buffer followed by centrifugation to remove cell debris and chromatography that removes endogenous amino acids and plant pigments from the extract. The extract is also treated with micrococcal nuclease to destroy endogenous mRNA, thus minimizing background translation.

The extract contains an energy-regenerating system (phosphocreatine/phosphocreatine kinase), spermidine to stimulate the efficiency of chain elongation, magnesium acetate and potassium acetate. Potassium Acetate enhances translation for a wide range of mRNAs. Three Amino Acid Mixtures are provided, which enable use of different radioisotopes.

For more information, see the Protein Expression chapter of the Protocols & Applications Guide.

Learn more about our custom options for this product at: www.promega.com/custom/

Applications
  • Expression of eukaryotic messages that do not express well in reticulocyte lysates
  • Expression of plant mRNAs
  • Expression of mammalian transcription factors

Protocols

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Wheat Germ Extract

L418A 5 x 200μl

Potassium Acetate

L420A 1 x 0.5ml1M

Amino Acid Mixture Minus Cysteine

L447A 1 x 175μl1mM View Product

Luciferase Control RNA

L456A 1 x 10μl View Product

Amino Acid Mixture Minus Leucine

L995B 1 x 175μl1mM View Product

Amino Acid Mixture Minus Methionine

L996B 1 x 175μl1mM View Product

SDS

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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