The TnT® T7 Quick for PCR DNA System is a simple, single-tube coupled transcription/translation system designed for optimum protein expression directly from PCR templates, no cleanup needed. For most PCR templates, the reactions produce up to 5 times more protein than other commercially available kits. The PCR-generated DNA can be used directly from the amplification reaction or purified by numerous commercially available kits and traditional methods.
Reagents including Recombinant RNasin® Ribonuclease Inhibitor are included in the Quick Master Mix.
For more information, see the Protein Expression chapter of the Protocols & Applications Guide.
- Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.