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TnT® T7 Quick for PCR DNA

Coupled Transcription/Translation System for Optimum Protein Expression from PCR Templates

  • Express protein directly from PCR, no cleanup needed
  • Up to 5 times more protein than standard translation reactions with linear templates
  • Includes Recombinant RNasin® Ribonuclease Inhibitor

Size

Catalog number selected: L5540

$ 600.00 Your price: Log In

TnT® T7 Quick for PCR DNA
40 reactions
$ 600.00
Your price: Log In

Protein from PCR Templates, No Clean Up Required

The TnT® T7 Quick for PCR DNA System is a simple, single-tube coupled transcription/translation system designed for optimum protein expression directly from PCR templates, no cleanup needed. For most PCR templates, the reactions produce up to 5 times more protein than other commercially available kits. The PCR-generated DNA can be used directly from the amplification reaction or purified by numerous commercially available kits and traditional methods.

Reagents including Recombinant RNasin® Ribonuclease Inhibitor are included in the Quick Master Mix.

For more information, see the Protein Expression chapter of the Protocols & Applications Guide.

Reference

  1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

Protocols

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Methionine

L118A 1 x 50μl1mM

TnT® T7 PCR Quick Master Mix

L554A 8 x 200μl

Nuclease-Free Water

P119A 1 x 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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