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TnT® SP6 High-Yield Wheat Germ Protein Expression System

L3260

Coupled Transcription/Translation for Soluble, Full-Length Protein in Only Two Hours

  • Express soluble, full-length protein from plasmid or PCR-generated templates quickly
  • Generate 10- to 20-fold more protein (10–100μg/ml) than with other cell-free systems

Size

Catalog number selected: L3260

$ 674.00 Your price: Log In

TnT® SP6 High-Yield Wheat Germ Protein Expression System
40 reactions
$ 674.00
Your price: Log In
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Generate Soluble, Full-Length Protein in Two Hours

The TnT® SP6 High-Yield Wheat Germ Protein Expression System contains optimized wheat germ extract in a single-tube, coupled transcription/translation system that allows you to express proteins in only two hours from plasmid or PCR-generated templates. Protein is synthesized in concentrations in the range of 10–100μg/ml and can be used in multiple applications including high-throughput analysis.

All components necessary for transcription/translation are provided in the extract, with the exception of the plasmid DNA or PCR template. Optional protein-labeling reagents must also be supplied by the user.

For more information, see the Protocols & Applications Guide.

For custom wheat germ extract (depleted amino acids), order Cat.# X808X (see Related Products).

Applications

  • Protein:protein interactions
  • Protein:nucleic acid interactions
  • Protein:ligand interactions
  • Enzyme assays
  • Protein expression analysis

References

  1. Falah, N. et al. (2012) J. Virol. 86, 691–704.
  2. Stempin, C.C. et al. (2011) J. Biol. Chem. 286, 37147–57.
  3. Liu, W. et al. (2011) Plant Cell. 23, 3853–65.
  4. Shi, X. et al. (2011) J. Biol. Chem. 286, 33401–8.
  5. Zhao, L. et al. (2010) J. Struct. Funct. Genomics 11, 201–9.
  6. Zhao, K.Q., Hurst, R., Slater, M.R. and Bulleit, R.F. (2007) J. Struct. Funct. Genomics 8, 199–208.

Protocols

Specifications

You are viewing: 40 reactions Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

TnT® SP6 High-Yield Wheat Germ Master Mix

L326A 4 x 300μl

Nuclease-Free Water

P119A 1 x 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: 10 reactions Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

TnT® SP6 High-Yield Wheat Germ Master Mix

L326A 1 x 300μl

Nuclease-Free Water

P119A 1 x 1,250μl View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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