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TnT® Quick Coupled Transcription/Translation System

L1170

Single-Tube, Coupled Transcription/Translation for Eukaryotic Cell-Free Protein Expression

  • Functional protein is expressed in one hour, ready for use without further purification
  • Use protein for protein:protein and protein:nucleic acid interactions, protein modification and more
  • Choice of SP6 or T7 promoter

Choose a Promoter

Size

Catalog number selected: L1170

$ 600.00 Your price: Log In

TnT® Quick Coupled Transcription/Translation System
T7/40 reactions
$ 600.00
Your price: Log In
Change Configuration

Express Protein from PCR Fragments or Plasmid DNA

The TnT® Quick Coupled Transcription/Translation Systems provide convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression. These cell-free expression systems combine the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin® Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TnT® Quick Master Mix.

The TnT® Quick Coupled Transcription/Translation System is available in two configurations for the expression of genes cloned downstream from either the T7 or SP6 RNA polymerase promoters. To use these cell-free expression systems, 0.2–2.0μg of circular plasmid DNA containing a T7 or SP6 promoter, or a PCR-generated fragment containing a T7 promoter, is added to an aliquot of the TnT® Quick Master Mix and incubated in a 50μl reaction volume for 60–90 minutes at 30°C. The expression reaction produces significant quantities of protein for a variety of applications including GST pull-downs and gel shift assays.

These mammalian-based systems express soluble, functional proteins that are post-translationally modified, unlike E. coli-based systems. Expressed proteins can be used directly after expression; no additional  protein purification needed. 

For more information about cell-free protein expression, see the Protein Expression chapter of the Protocols & Applications Guide.

Applications

  • GST pull-downs (view an animation).
  • Gel shift assays (view an animation).
  • Co-immunoprecipitation (view an animation).
  • Characterization of protein modifications (view an animation).
  • Protein:RNA interactions.
  • Protein activity studies.
  • Confirmation of gene products.
  • Protein Truncation Test (PTT).
Schematic diagram illustrating how the TNT® Quick System functions.

Schematic showing DNA to protein expression in 60–90 minutes.

Protocols

Specifications

You are viewing: L1170 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Methionine

L118A 1 × 50μl1mM

T7 TnT® PCR Enhancer

L216A 1 × 100μl

Luciferase T7 Control DNA

L482A 1 × 5μg0.5μg/μl

Luciferase Assay Reagent

L486A 1 × 250μl

TnT® T7 Quick Master Mix

L520A 8 × 200μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L1171 Change Configuration

What's in the box?

Item Part # SizeConcentration

Methionine

L118A 1 × 50μl1mM

T7 TnT® PCR Enhancer

L216A 1 × 100μl

Luciferase T7 Control DNA

L482A 1 × 5μg0.5μg/μl

TnT® T7 Quick Master Mix

L520A 1 × 200μl

SDS

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Certificate of Analysis

Search for Specific Certificate:

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L2080 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Methionine

L118A 1 × 50μl1mM

TnT® SP6 Quick Master Mix

L209A 8 × 200μl

Luciferase SP6 Control DNA

L474A 1 × 5μg0.5μg/μl

Luciferase Assay Reagent

L486A 1 × 250μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L2081 Change Configuration

What's in the box?

Item Part # SizeConcentration

Methionine

L118A 1 × 50μl1mM

TnT® SP6 Quick Master Mix

L209A 1 × 200μl

Luciferase SP6 Control DNA

L474A 1 × 5μg0.5μg/μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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