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TnT® Coupled Wheat Germ Extract System

Single-Tube Coupled Transcription/Translation System

  • Produces 2- to 6-fold more protein than standard wheat germ systems
  • Produce protein from linearized DNA in 1.5 hours
  • You choose the promoter

Choose a Promoter

Size

Catalog number selected: L4130

$ 552.00 Your price: Log In

TnT® Coupled Wheat Germ Extract System
SP6/40 reactions
$ 552.00
Your price: Log In
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Transcription and Translation in a Single Tube

The TnT® Coupled Wheat Germ Extract Systems offer an alternative for eukaryotic cell-free protein expression: a one-tube, coupled transcription/translation system. The TnT® Extract Systems greatly simplify the process and reduce the time required to obtain in vitro translation results. Standard wheat germ extract translations commonly use RNA synthesized in vitro from SP6 or T7 RNA polymerase promoters. This entire process requires separate reactions with several steps between each reaction. The TnT® Extracts bypass many of these steps by incorporating transcription directly in the translation mix. Additionally, the TnT® Extract reactions often produce significantly more protein (two- to sixfold) in a 1.5-hour reaction than do standard in vitro wheat germ extract translations using RNA templates.

Magnesium Acetate, 25mM, and Potassium Chloride, 2.5M, can be used to optimize in vitro translation reactions in the TnT® T7 Quick Coupled Transcription/Translation System, Flexi® Rabbit Reticulocyte Lysate System and TnT® Coupled Wheat Germ Extract System.

To produce protein from linear plasmids, choose the T7 promoter. The SP6 promoter will produce protein from circular DNA. For PCR templates use TnT® T7 Quick for PCR DNA (Cat.# L5540). Luciferase Control DNA and Luciferase Assay Reagents are included with the system as functional controls. Only full-length luciferase is active.

For more information, see the Protein Expression chapter of the Protocols & Applications Guide.

Applications

  • Protein:protein interactions.
  • Protein activity studies.
  • The Wheat Germ Extract System is particularly suited to translation of mammalian transcription factors, since it does not contain mammalian transcription factors.
  • Protein:nucleic acid interactions.
  • Simultaneously express multiple genes that are under the control of different phage polymerase promoters.
  • Rapid screening and analysis of mutants.
  • Protein Truncation Test (PTT).

References

  1. Anderson, C.W. et al. (1983) Meth. Enzymol. 101, 635–44.
  2. Krieg, P.A. and Melton, D.A. (1984) Nucl. Acids Res. 12, 7057–70.

Protocols

Specifications

You are viewing: 40 reactions Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

TnT® Wheat Germ Extract

L411A 5 x 200μl

Amino Acid Mixture Minus Cysteine

L447C 1 x 50μl1mM View Product

Luciferase Assay Wells

L457A 1 x 3 each

TnT® Reaction Buffer

L462A 1 x 90μl

Luciferase SP6 Control DNA

L474A 1 x 5μg0.5μg/μl

Luciferase Assay Reagent

L486A 1 x 250μl

TnT® SP6 Wheat Germ Polymerase

L515A 1 x 60μl

Amino Acid Mixture Minus Leucine

L995A 1 x 50μl1mM View Product

Amino Acid Mixture Minus Methionine

L996A 1 x 50μl1mM View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Specifications

You are viewing: 40 reactions Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

TnT® Wheat Germ Extract

L411A 5 x 200μl

Amino Acid Mixture Minus Cysteine

L447C 1 x 50μl1mM View Product

Luciferase Assay Wells

L457A 1 x 3 each

TnT® Reaction Buffer

L462A 1 x 90μl

Luciferase Assay Reagent

L486A 1 x 250μl

TnT® T7 Wheat Germ Polymerase

L516A 1 x 60μl

Amino Acid Mixture Minus Leucine

L995A 1 x 50μl1mM View Product

Amino Acid Mixture Minus Methionine

L996A 1 x 50μl1mM View Product

T7 Linear Control DNA

P141A 1 x 10μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

Specifications

You are viewing: 40 reactions Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

TnT® Wheat Germ Extract

L411A 5 x 200μl

Amino Acid Mixture Minus Cysteine

L447C 1 x 50μl1mM View Product

Luciferase Assay Wells

L457A 1 x 3 each

TnT® Reaction Buffer

L462A 1 x 90μl

Luciferase SP6 Control DNA

L474A 1 x 5μg0.5μg/μl

Luciferase Assay Reagent

L486A 1 x 250μl

TnT® SP6 Wheat Germ Polymerase

L515B 1 x 30μl

TnT® T7 Wheat Germ Polymerase

L516B 1 x 30μl

Amino Acid Mixture Minus Leucine

L995A 1 x 50μl1mM View Product

Amino Acid Mixture Minus Methionine

L996A 1 x 50μl1mM View Product

T7 Linear Control DNA

P141A 1 x 10μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

See Protocol for detailed storage recommendations.

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