We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Regulated Mammalian Expression System

Modulate Protein Expression in Mammalian Cells

  • Dose-response induction of protein expression
  • Rapid, responsive on/off protein expression switch

Choose a Product

Size

Catalog number selected: C9470

$ 759.00 Your price: Log In

Add to Helix
Regulated Mammalian Expression System
Regulated Mammalian Expression System/1 system
$ 759.00
Your price: Log In
Change Configuration

Rapid Induction and Downregulation of Mammalian Gene Expression

Features low basal levels, robust and rapid induction, and downregulation of gene expression in mammalian cells. The Regulated Mammalian Expression System is based on a novel on/off switch that relies on the rapid and sensitive modulation by coumerin-related compounds of a chimeric transactivator protein. Nanomolar concentrations of the antibiotic coumermycin promote homodimerization of a chimeric transactivator that, in turn, binds to lambda operator sequences located upstream of a minimal promoter, driving transcription of coding sequences for a protein of interest. The level of protein expression can be regulated by adjusting the coumermycin concentration. More significantly, this expression can be promptly and effectively switched off by adding novobiocin, which acts as an antagonist by dissociating the dimerized transactivator protein.

The protein coding region of interest is cloned into either the pF12A RM Flexi® Vector or pF12K RM Flexi® Vector, both of which are specially designed for Regulated Mammalian (RM) protein expression. These vectors incorporate regulatory promoter sequences upstream of the protein-coding region and are compatible with the Flexi® Vector System. In transient transfection paradigms, the pF12A or pF12K RM Flexi® Vector containing the protein-coding region of interest is co-transfected into mammalian cells together with the pReg neo Vector. The pReg neo Vector is designed to express a chimeric transactivator protein that interacts with the regulatory promoter region in the pF12A and pF12K RM Flexi® Vectors in a regulated fashion in response to coumermycin and novobiocin. Additionally, the pReg neo Vector encodes a neomycin phosphotransferase gene that allows stable cell selection and generation with the antibiotic G-418.

Application
  • Regulated protein expression in mammalian cells.
Reference
  1. Zhao, H-F. et al. (2003) Hum. Gene Ther. 14, 1619–29.

GenBank®/EMBL Accession Numbers and Sequence Information

pReg neo Vector Accession Number EF030522; pReg neo Vector sequence text file.
pF12A RM Flexi® Vector Accession Number EF030520; pF12A RM Flexi® Vector sequence text file.
pF12K RM Flexi® Vector Accession Number EF030521; pF12K RM Flexi®Vector sequence text file.

Representation of the coumerin-regulated system.

Schematic of coumermycin-regulated system.

6066MA.eps
pReg neo Vector.
6068MA.eps
pF12A RM Flexi® Vector.
6067MA.eps
pF12K RM Flexi® Vector.

Protocols

Specifications

You are viewing: 1 system Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

pReg neo Vector

C942A 1 x 20μg View Product

pF12A RM Flexi® Vector

C943A 1 x 20μg View Product

pF12K RM Flexi® Vector

C944A 1 x 20μg View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.

Researchers may use this product in their own research and they may transfer derivatives to others for research use provided third parties agree to be bound by the terms of this label license. No commercial use is allowed. Commercial use means any and all uses of this product or derivatives by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, please contact Promega for licensing information. No other use or transfer of this product or derivatives is authorized without the prior express, written consent of Promega. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,293,503 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

Patent Pending.

For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

Licensed from National Research Council of Canada.

Specifications

You are viewing: 5mg Change Configuration

What's in the box?

Item Part # Size

Coumermycin A1

C945A 1 x 5mg

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: 1g Change Configuration

What's in the box?

Item Part # Size

Novobiocin Sodium Salt

C946A 1 x 1g

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Therapeutic or Diagnostic Procedures.

Storage Conditions

Resources

Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom