This system contains both Rabbit Reticulocyte Lysate and Wheat Germ Extract for comparing in vitro translation systems. Reticulocyte Lysate is prepared from New Zealand white rabbits. The Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cellular debris. Both systems contain the cellular components necessary for protein synthesis. The systems have been treated with micrococcal nuclease, which destroys endogenous mRNA and results in minimal background translation.
Each system contains 2 × 200μl of both Rabbit Reticulocyte Lysate and Wheat Germ Extract; this is sufficient for 12 reactions using the Rabbit Reticulocyte Lysate and 12 reactions using the Wheat Germ Extract. Test both the lysate and extract to find the optimal translation system for your studies. Rigorous quality control ensures minimal lot-to-lot variability. Potassium Acetate is provided to enable use of Wheat Germ Extract with a wide range of mRNAs.
Learn more: See the Protein Expression chapter of the Protocols & Applications Guide.
- Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.