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Rabbit Reticulocyte Lysate System, Nuclease Treated

In Vitro Translation of mRNA and Characterization of mRNA Products

  • Optimized for translation and ready to use
  • Contains an energy-regenerating system, a mix of tRNAs, hemin, potassium chloride and magnesium acetate

Size

Catalog number selected: L4960

$ 197.00 Your price: Log In

Rabbit Reticulocyte Lysate System, Nuclease Treated
30 reactions
$ 197.00
Your price: Log In

Identify mRNAs, Characterize Protein Products 

Rabbit Reticulocyte Lysate System, Nuclease Treated can be used to identify mRNA species, characterize their protein products and investigate transcriptional and translational control. Rabbit Reticulocyte Lysate is prepared from New Zealand white rabbits using a standard protocol that ensures reliable and consistent reticulocyte production in each lot. After the reticulocytes are lysed, the extract is treated with micrococcal nuclease to destroy endogenous mRNA and thus reduce background translation to a minimum. The lysate contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors).

Learn More: See the Protein Expression chapter of the Protocols & Applications Guide for more information.

Choose Your Configuration: Learn more about custom options for this product at: www.promega.com/custom/

Applications
  • In vitro translation from mRNA, in vitro run-off transcripts or total RNA into protein
  • Characterization of mRNA products
  • Co-translational processing of secreted proteins by the addition of microsomal membranes to the translation reaction
References
  1. Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.
  2. Glass, C.A. and Pollard, K.M. (1990) Promega Notes 26, 6–9.

Protocols

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Reticulocyte Lysate, Nuclease Treated

L416A 5 x 200μl

Amino Acid Mixture Minus Cysteine

L447C 1 x 50μl1mM View Product

Luciferase Control RNA

L456A 1 x 10μl View Product

Amino Acid Mixture Minus Leucine

L995A 1 x 50μl1mM View Product

Amino Acid Mixture Minus Methionine

L996A 1 x 50μl1mM View Product

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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