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The TnT® SP6 High-Yield Wheat Germ Protein Expression System, based on an optimized wheat germ extract, is a single-tube, coupled transcription/translation system designed to express proteins in only two hours. Protein synthesized, in the range of 10–100μg/ml, can be used in multiple proteomic-based applications, as well as in high-throughput analysis.
All components necessary for transcription/translation are provided in the extract, with the exception of the plasmid DNA or PCR template. Optional protein-labeling reagents must also be supplied by the user.
For custom wheat germ extract (depleted amino aci...
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For custom wheat germ extract (depleted amino acids), order Cat.# X808X (see Products, Available Separately).
For more information, see the Protocols & Applications Guide.
TnT® SP6 High-Yield Wheat Germ Protein Expression System
TnT® SP6 High-Yield Wheat Germ Master Mix
TnT® SP6 High-Yield Master Mix Minus Amino Acids
Store at –70°C.
For product intended use please see Patents & Disclaimers tab.
Proteins were expressed by dialysis reaction using 8µg of plasmid DNA. Lane 1, firefly luciferase (62kDa); lane 2, GFP (28kDa); lane 3, Renilla luciferase (36kDa).
L3260, L3261, X808X For Research Use Only. Not for Use in Diagnostic Procedures.
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