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S30 T7 High-Yield Protein Expression System

Simplified Transcription/Translation of DNA Cloned in Plasmid or Lambda Vectors with a T7 Promoter

  • Express up to 500μg/ml of protein in one hour
  • Flexible detection of expressed proteins by Coomassie® staining or fluorescence or biotinylated labels

Size

Catalog number selected: L1110

$ 509.00
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S30 T7 High-Yield Protein Expression System
24 reactions
$ 509.00
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High-Yield Protein Expression in One Hour 

With the S30 T7 High-Yield Protein Expression System you can express up to 500μg/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site. The protein expression system provides an extract that contains T7 RNA polymerase for transcription and is deficient in OmpT endoproteinase and lon protease activity. All other necessary components in the system are optimized for protein expression. This results in greater stability and enhanced expression of target proteins.

Detect expressed proteins by Coomassie® staining or incorporation of a fluorescence or biotinylated modified tRNA. For more information, see the Protocols & Applications Guide.

Applications
  • Protein:protein interactions
  • Protein:DNA interactions
  • Enzyme activity
  • Antibody production
9695MF_500

Schematic of the protein expression system.

Specifications

You are viewing: L1110 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

S30 Premix Plus

L111A 3 × 200μl

S30 T7 Control DNA

L112A 1 × 20μl

T7/S30 Extract for Circular DNA

L114A 3 × 150μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

SDS

Choose language:

Download SDSPDF (174 KB) – English (United States)

Certificate of Analysis

See all certificates for L1110

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

Specifications

You are viewing: L1115 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

S30 Premix Plus

L111A 1 × 200μl

S30 T7 Control DNA

L112A 1 × 20μl

T7/S30 Extract for Circular DNA

L114A 1 × 150μl

Nuclease-Free Water

P119A 1 × 1,250μl View Product

SDS

Choose language:

Download SDSPDF (176 KB) – English (United States)

Certificate of Analysis

See all certificates for L1115

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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