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Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System

Contains all Components Needed for Protein Synthesis

  • Enables testing with Rabbit Reticulocyte Lysate and Wheat Germ Systems to optimize translation
  • Can be used to express a wide range of mRNAs

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Size

Catalog number selected: L4330

$ 249.00
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This product is discontinued
Rabbit Reticulocyte Lysate/Wheat Germ Extract Combination System
24 reactions
$ 249.00
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Compare to Optimize Your Translation Studies

This system contains both Rabbit Reticulocyte Lysate and Wheat Germ Extract for comparing in vitro translation systems. Reticulocyte Lysate is prepared from New Zealand white rabbits. The Wheat Germ Extract is prepared by grinding wheat germ in an extraction buffer followed by centrifugation to remove cellular debris. Both systems contain the cellular components necessary for protein synthesis. The systems have been treated with micrococcal nuclease, which destroys endogenous mRNA and results in minimal background translation.

Each system contains 2 × 200μl of both Rabbit Reticulocyte Lysate and Wheat Germ Extract; this is sufficient for 12 reactions using the Rabbit Reticulocyte Lysate and 12 reactions using the Wheat Germ Extract. Test both the lysate and extract to find the optimal translation system for your studies. Rigorous quality control ensures minimal lot-to-lot variability. Potassium Acetate is provided to enable use of Wheat Germ Extract with a wide range of mRNAs.

Learn more: See the Protein Expression chapter of the Protocols & Applications Guide.

Reference

  1. Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Reticulocyte Lysate, Nuclease Treated

L416A 2 × 200μl

Wheat Germ Extract

L418A 2 × 200μl

Potassium Acetate

L420A 1 × 0.5ml1M

Amino Acid Mixture Minus Cysteine

L447A 1 × 175μl1mM View Product

Luciferase Control RNA

L456A 1 × 10μl View Product

Amino Acid Mixture Minus Leucine

L995B 1 × 175μl1mM View Product

Amino Acid Mixture Minus Methionine

L996B 1 × 175μl1mM View Product

SDS

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Download SDSPDF (253 KB) – English (United States)

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

AA

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Manas

India