pF12K RM Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pF12K RM Flexi(R) Vector sequence reference points: Base pairs 3465 12X lambda operator 16-309 Minimal CMV promoter 322-434 SgfI site 446-453 Barnase coding region 477-812 PmeI site 814-821 SV40 late poly(A) signal 973-1194 Kanamycin resistance gene 1502-2296 ColE1-derived origin of replication 2465-2501 cer site (site for E. coli XerCD recombinase) 3172-3457 .. 1 AGTACTCCGC TCGAGTTTAC CTCTGGCGGT GATAGTCGAG TTTACCTCTG 51 GCGGTGATAG TCGAGTTTAC CTCTGGCGGT GATAGTCGAG TTTACCTCTG 101 GCGGTGATAG TCGAGTTTAC CTCTGGCGGT GATAGTCGAG TTTACCTCTG 151 GCGGTGATAG TCGAGTTTAC CTCTGGCGGT GATAGTCGAG TTTACCTCTG 201 GCGGTGATAG TCGAGTTTAC CTCTGGCGGT GATAGTCGAG TTTACCTCTG 251 GCGGTGATAG TCGAGTTTAC CTCTGGCGGT GATAGTCGAG TTTACCTCTG 301 GCGGTGATAG TCGACTCTAG ATAGGCGTGT ACGGTGGGAG GCCTATATAA 351 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC CTGGAGACGC CATCCACGCT 401 GTTTTGACCT CCATAGAAGA CACCGGGACC GATCAACCTA AGCTTGCGAT 451 CGCCATGGAA TAAGTAAGGA ATCCACATGG CACAGGTTAT CAACACGTTT 501 GACGGGGTTG CGGATTATCT TCAGACATAT CATAAGCTAC CTGATAATTA 551 CATTACAAAA TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA TCAAAAGGGA 601 ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA CATCTTCTCA 651 AACAGGGAAG GCAAACTCCC GGGCAAAAGC GGACGAACAT GGCGTGAAGC 701 GGATATTAAC TATACATCAG GCTTCAGAAA TTCAGACCGG ATTCTTTACT 751 CAAGCGACTG GCTGATTTAC AAAACAACGG ACCATTATCA GACCTTTACA 801 AAAATCAGAT AATGTTTAAA CGAATTCGGG CTCGGTACCC GGGGATCCTC 851 TAGAGTCGAC CTGCAGGCAT GCAAGCTGAT CCGGCTGCTA ACAAAGCCCG 901 AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC 951 CCCTTGGGGC GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT 1001 TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA 1051 TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA 1101 GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGAT 1151 GTGGGAGGTT TTTTTAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG 1201 AATTCTAATG GATCCTCTTT GCGCTTGCGT TTTCCCTTGT CCAGATAGCC 1251 CAGTAGCTGA CATTCATCCG GGGTCAGCAC CGTTTCTGCG GACTGGCTTT 1301 CTACGTGTTC CGCTTCCTTT AGCAGCCCTT GCGCCCTGAG TGCTTGCGGC 1351 AGCGTGAGCT TCAAAAGAAT TGCCAGCTGG GGCGCCCTCT GGTAAGGTTG 1401 GGAAGCCCTG CAAAGTAAAC TGGATGGCTT TCTTGCCGCC AAGGATCTGA 1451 TGGCGCAGGG GATCAAGATC TGATCAAGAG ACAGGATGAC GGTCGTTTCG 1501 CATGCTTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG 1551 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 1601 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA 1651 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC 1701 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT 1751 GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA 1801 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG 1851 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 1901 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGCACTC GGATGGAAGC 1951 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC 2001 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GTATGCCGGA TGGTGAGGAT 2051 CTCGTCGTGA CTCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA 2101 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC 2151 GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 2201 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA 2251 TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG 2301 GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CGGTATCAGC 2351 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 2401 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 2451 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 2501 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 2551 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 2601 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 2651 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 2701 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 2751 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 2801 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 2851 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 2901 ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 2951 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 3001 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 3051 TTTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 3101 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 3151 TCACCTAGAT CCTTTTATAG TCCGGAAATA CAGGAACGCA CGCTGGATGG 3201 CCCTTCGCTG GGATGGTGAA ACCATGAAAA ATGGCAGCTT CAGTGGATTA 3251 AGTGGGGGTA ATGTGGCCTG TACCCTCTGG TTGCATAGGT ATTCATACGG 3301 TTAAAATTTA TCAGGCGCGA TTGCGGCAGT TTTTCGGGTG GTTTGTTGCC 3351 ATTTTTACCT GTCTGCTGCC GTGATCGCGC TGAACGCGTT TTAGCGGTGC 3401 GTACAATTAA GGGATTATGG TAAATCCACT TACTGTCTGC CCTCGTAGCC 3451 ATCGAGATAA ACCGC Sequence file prepared 2-Mar-07.