Promega's Cookie Policy

We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

Regulated Mammalian Expression System

We are sorry, but this product is discontinued.

For more information, contact Technical Service.

Catalog number selected: C9451

Please Enquire
This product is discontinued
Regulated Mammalian Expression System

Rapid Induction and Downregulation of Mammalian Gene Expression

Features low basal levels, robust and rapid induction, and downregulation of gene expression in mammalian cells. The Regulated Mammalian Expression System is based on a novel on/off switch that relies on the rapid and sensitive modulation by coumerin-related compounds of a chimeric transactivator protein. Nanomolar concentrations of the antibiotic coumermycin promote homodimerization of a chimeric transactivator that, in turn, binds to lambda operator sequences located upstream of a minimal promoter, driving transcription of coding sequences for a protein of interest. The level of protein expression can be regulated by adjusting the coumermycin concentration. More significantly, this expression can be promptly and effectively switched off by adding novobiocin, which acts as an antagonist by dissociating the dimerized transactivator protein.

The protein coding region of interest is cloned into either the pF12A RM Flexi® Vector or pF12K RM Flexi® Vector, both of which are specially designed for Regulated Mammalian (RM) protein expression. These vectors incorporate regulatory promoter sequences upstream of the protein-coding region and are compatible with the Flexi® Vector System. In transient transfection paradigms, the pF12A or pF12K RM Flexi® Vector containing the protein-coding region of interest is co-transfected into mammalian cells together with the pReg neo Vector. The pReg neo Vector is designed to express a chimeric transactivator protein that interacts with the regulatory promoter region in the pF12A and pF12K RM Flexi® Vectors in a regulated fashion in response to coumermycin and novobiocin. Additionally, the pReg neo Vector encodes a neomycin phosphotransferase gene that allows stable cell selection and generation with the antibiotic G-418.

Application
  • Regulated protein expression in mammalian cells.
Reference
  1. Zhao, H-F. et al. (2003) Hum. Gene Ther. 14, 1619–29.

GenBank®/EMBL Accession Numbers and Sequence Information

pReg neo Vector Accession Number EF030522; pReg neo Vector sequence text file.
pF12A RM Flexi® Vector Accession Number EF030520; pF12A RM Flexi® Vector sequence text file.
pF12K RM Flexi® Vector Accession Number EF030521; pF12K RM Flexi®Vector sequence text file.

Representation of the coumerin-regulated system.

Schematic of coumermycin-regulated system.

pReg neo Vector.

pReg neo Vector.
pReg neo Vector.

pF12A RM Flexi® Vector.

pF12A RM Flexi® Vector.
pF12A RM Flexi® Vector.

pF12K RM Flexi® Vector.

pF12K RM Flexi® Vector.
pF12K RM Flexi® Vector.

Specifications

You are viewing: C9470 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

pReg neo Vector

C942A 1 × 20μg View Product

pF12A RM Flexi® Vector

C943A 1 × 20μg View Product

pF12K RM Flexi® Vector

C944A 1 × 20μg View Product

SDS

Choose language:

Download SDSPDF (232 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: C9451 Change Configuration

What's in the box?

Item Part # Size

Coumermycin A1

C945A 1 × 5mg

SDS

Choose language:

Download SDSPDF (188 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

Specifications

You are viewing: C9461 Change Configuration

What's in the box?

Item Part # Size

Novobiocin Sodium Salt

C946A 1 × 1g

SDS

Choose language:

Download SDSPDF (194 KB) – English (United States)

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Therapeutic or Diagnostic Procedures.

Storage Conditions

CC

Let's find the product that meets your needs.

Talk to a Scientist

Luca

Luca

Italy