pFN23K (HaloTag(R)) CMVd2 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN23K (HaloTag(R)) CMVd2 Flexi(R) Vector sequence reference points: Base pairs 4135 CMVd2 intermediate early enhancer/promoter 1-73 T7 RNA polymerase promoter (-17 to +3) 84-103 SP6 RNA polymerase promoter (-17 to +3) 108-127 HaloTag(R) 7 protein coding region 143-1033 TEV region 1046-1066 SgfI region 1073-1080 Barnase coding region 1104-1439 PmeI region 1441-1448 SV40 late polyadenylation signal 1600-1821 Kanamycin resistance (Kanr) coding region 2129-2923 ColE1-derived plasmid origin of replication 3092-3128 cer region (for E. coli XerCD recombinase) 3799-4084 .. 1 GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG 51 CTGGTTTAGT GAACCGTCAG ATCACTAGAA GCTTAATACG ACTCACTATA 101 GGGTACGATT TAGGTGACAC TATAGAATAA GGAGAATTCA TCATGGCAGA 151 AATCGGTACT GGCTTTCCAT TCGACCCCCA TTATGTGGAA GTCCTGGGCG 201 AGCGCATGCA CTACGTCGAT GTTGGTCCGC GCGATGGCAC CCCTGTGCTG 251 TTCCTGCACG GTAACCCGAC CTCCTCCTAC GTGTGGCGCA ACATCATCCC 301 GCATGTTGCA CCGACCCATC GCTGCATTGC TCCAGACCTG ATCGGTATGG 351 GCAAATCCGA CAAACCAGAC CTGGGTTATT TCTTCGACGA CCACGTCCGC 401 TTCATGGATG CCTTCATCGA AGCCCTGGGT CTGGAAGAGG TCGTCCTGGT 451 CATTCACGAC TGGGGCTCCG CTCTGGGTTT CCACTGGGCC AAGCGCAATC 501 CAGAGCGCGT CAAAGGTATT GCATTTATGG AGTTCATCCG CCCTATCCCG 551 ACCTGGGACG AATGGCCAGA ATTTGCCCGC GAGACCTTCC AGGCCTTCCG 601 CACCACCGAC GTCGGCCGCA AGCTGATCAT CGATCAGAAC GTTTTTATCG 651 AGGGTACGCT GCCGATGGGT GTCGTCCGCC CGCTGACTGA AGTCGAGATG 701 GACCATTACC GCGAGCCGTT CCTGAATCCT GTTGACCGCG AGCCACTGTG 751 GCGCTTCCCA AACGAGCTGC CAATCGCCGG TGAGCCAGCG AACATCGTCG 801 CGCTGGTCGA AGAATACATG GACTGGCTGC ACCAGTCCCC TGTCCCGAAG 851 CTGCTGTTCT GGGGCACCCC AGGCGTTCTG ATCCCACCGG CCGAAGCCGC 901 TCGCCTGGCC AAAAGCCTGC CTAACTGCAA GGCTGTGGAC ATCGGCCCGG 951 GTCTGAATCT GCTGCAAGAA GACAACCCGG ACCTGATCGG CAGCGAGATC 1001 GCGCGCTGGC TGTCGACGCT CGAGATTTCC GGCGAGCCAA CCACTGAGGA 1051 TCTGTACTTT CAGAGCGATA ACGCGATCGC CATGGAATAA GTAAGGAATC 1101 CACATGGCAC AGGTTATCAA CACGTTTGAC GGGGTTGCGG ATTATCTTCA 1151 GACATATCAT AAGCTACCTG ATAATTACAT TACAAAATCA GAAGCACAAG 1201 CCCTCGGCTG GGTGGCATCA AAAGGGAACC TTGCAGACGT CGCTCCGGGG 1251 AAAAGCATCG GCGGAGACAT CTTCTCAAAC AGGGAAGGCA AACTCCCGGG 1301 CAAAAGCGGA CGAACATGGC GTGAAGCGGA TATTAACTAT ACATCAGGCT 1351 TCAGAAATTC AGACCGGATT CTTTACTCAA GCGACTGGCT GATTTACAAA 1401 ACAACGGACC ATTATCAGAC CTTTACAAAA ATCAGATAAT GTTTAAACGA 1451 ATTCGGGCTC GGTACCCGGG GATCCTCTAG AGTCGACCTG CAGGCATGCA 1501 AGCTGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG 1551 CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCGGC CGCTTCGAGC 1601 AGACATGATA AGATACATTG ATGAGTTTGG ACAAACCACA ACTAGAATGC 1651 AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT 1701 GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA 1751 TTTTATGTTT CAGGTTCAGG GGGAGATGTG GGAGGTTTTT TTAAGCAAGT 1801 AAAACCTCTA CAAATGTGGT AAAATCGAAT TCTAATGGAT CCTCTTTGCG 1851 CTTGCGTTTT CCCTTGTCCA GATAGCCCAG TAGCTGACAT TCATCCGGGG 1901 TCAGCACCGT TTCTGCGGAC TGGCTTTCTA CGTGTTCCGC TTCCTTTAGC 1951 AGCCCTTGCG CCCTGAGTGC TTGCGGCAGC GTGAGCTTCA AAAGAATTGC 2001 CAGCTGGGGC GCCCTCTGGT AAGGTTGGGA AGCCCTGCAA AGTAAACTGG 2051 ATGGCTTTCT TGCCGCCAAG GATCTGATGG CGCAGGGGAT CAAGATCTGA 2101 TCAAGAGACA GGATGACGGT CGTTTCGCAT GCTTGAACAA GATGGATTGC 2151 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 2201 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC 2251 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA 2301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC 2351 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG 2401 GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG 2451 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 2501 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT 2551 CGAGCGAGCA CGCACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC 2601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC 2651 AAGGCGCGTA TGCCGGATGG TGAGGATCTC GTCGTGACTC ATGGCGATGC 2701 CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG 2751 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT 2801 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT 2851 CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC 2901 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG 2951 ACCAAGCGAC GCCCAACCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 3001 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 3051 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 3101 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 3151 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA 3201 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 3251 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 3301 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 3351 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 3401 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 3451 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 3501 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT 3551 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 3601 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 3651 CAGCAGATTA CGCGCAGAAA AAAAGGATTT CAAGAAGATC CTTTGATCTT 3701 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 3751 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTATAGTCC 3801 GGAAATACAG GAACGCACGC TGGATGGCCC TTCGCTGGGA TGGTGAAACC 3851 ATGAAAAATG GCAGCTTCAG TGGATTAAGT GGGGGTAATG TGGCCTGTAC 3901 CCTCTGGTTG CATAGGTATT CATACGGTTA AAATTTATCA GGCGCGATTG 3951 CGGCAGTTTT TCGGGTGGTT TGTTGCCATT TTTACCTGTC TGCTGCCGTG 4001 ATCGCGCTGA ACGCGTTTTA GCGGTGCGTA CAATTAAGGG ATTATGGTAA 4051 ATCCACTTAC TGTCTGCCCT CGTAGCCATC GAGATAAACC GCAGTACTCC 4101 GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCT File updated 28-Apr-08.