pFN22A (HaloTag(R) 7) CMVd1 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN22A (HaloTag(R) 7) CMVd1 Flexi(R) Vector sequence reference points: Base pairs 4188 CMVd1 intermediate early enhancer/promoter 1-121 T7 RNA polymerase promoter (-17 to +3) 132-151 SP6 polymerase promoter (-17 to +3) 156-175 HaloTag(R) 7 protein coding region 191-1081 TEV region 1094-1114 SgfI region 1121-1128 Barnase coding region 1152-1487 Pme I region 1489-1496 SV40 late polyadenylation signal 1648-1869 Beta-lactamase (Ampr) coding region 2136-2990 ColE1-derived plasmid origin of replication 3145-3181 cer region (for E. coli XerCD recombinase) 3852-4137 .. 1 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAATAACCCC GCCCCGTTGA 51 CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT 101 GGTTTAGTGA ACCGTCAGAT CACTAGAAGC TTAATACGAC TCACTATAGG 151 GTACGATTTA GGTGACACTA TAGAATAAGG AGAATTCATC ATGGCAGAAA 201 TCGGTACTGG CTTTCCATTC GACCCCCATT ATGTGGAAGT CCTGGGCGAG 251 CGCATGCACT ACGTCGATGT TGGTCCGCGC GATGGCACCC CTGTGCTGTT 301 CCTGCACGGT AACCCGACCT CCTCCTACGT GTGGCGCAAC ATCATCCCGC 351 ATGTTGCACC GACCCATCGC TGCATTGCTC CAGACCTGAT CGGTATGGGC 401 AAATCCGACA AACCAGACCT GGGTTATTTC TTCGACGACC ACGTCCGCTT 451 CATGGATGCC TTCATCGAAG CCCTGGGTCT GGAAGAGGTC GTCCTGGTCA 501 TTCACGACTG GGGCTCCGCT CTGGGTTTCC ACTGGGCCAA GCGCAATCCA 551 GAGCGCGTCA AAGGTATTGC ATTTATGGAG TTCATCCGCC CTATCCCGAC 601 CTGGGACGAA TGGCCAGAAT TTGCCCGCGA GACCTTCCAG GCCTTCCGCA 651 CCACCGACGT CGGCCGCAAG CTGATCATCG ATCAGAACGT TTTTATCGAG 701 GGTACGCTGC CGATGGGTGT CGTCCGCCCG CTGACTGAAG TCGAGATGGA 751 CCATTACCGC GAGCCGTTCC TGAATCCTGT TGACCGCGAG CCACTGTGGC 801 GCTTCCCAAA CGAGCTGCCA ATCGCCGGTG AGCCAGCGAA CATCGTCGCG 851 CTGGTCGAAG AATACATGGA CTGGCTGCAC CAGTCCCCTG TCCCGAAGCT 901 GCTGTTCTGG GGCACCCCAG GCGTTCTGAT CCCACCGGCC GAAGCCGCTC 951 GCCTGGCCAA AAGCCTGCCT AACTGCAAGG CTGTGGACAT CGGCCCGGGT 1001 CTGAATCTGC TGCAAGAAGA CAACCCGGAC CTGATCGGCA GCGAGATCGC 1051 GCGCTGGCTG TCGACGCTCG AGATTTCCGG CGAGCCAACC ACTGAGGATC 1101 TGTACTTTCA GAGCGATAAC GCGATCGCCA TGGAATAAGT AAGGAATCCA 1151 CATGGCACAG GTTATCAACA CGTTTGACGG GGTTGCGGAT TATCTTCAGA 1201 CATATCATAA GCTACCTGAT AATTACATTA CAAAATCAGA AGCACAAGCC 1251 CTCGGCTGGG TGGCATCAAA AGGGAACCTT GCAGACGTCG CTCCGGGGAA 1301 AAGCATCGGC GGAGACATCT TCTCAAACAG GGAAGGCAAA CTCCCGGGCA 1351 AAAGCGGACG AACATGGCGT GAAGCGGATA TTAACTATAC ATCAGGCTTC 1401 AGAAATTCAG ACCGGATTCT TTACTCAAGC GACTGGCTGA TTTACAAAAC 1451 AACGGACCAT TATCAGACCT TTACAAAAAT CAGATAATGT TTAAACGAAT 1501 TCGGGCTCGG TACCCGGGGA TCCTCTAGAG TCGACCTGCA GGCATGCAAG 1551 CTGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC 1601 ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCGGCCG CTTCGAGCAG 1651 ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG 1701 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT 1751 AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT 1801 TTATGTTTCA GGTTCAGGGG GAGATGTGGG AGGTTTTTTT AAGCAAGTAA 1851 AACCTCTACA AATGTGGTAA AATCGAATTC TAATGGATCC TCTTTGCGCT 1901 TGCGTTTTCC CTTGTCCAGA TAGCCCAGTA GCTGACATTC ATCCGGGGTC 1951 AGCACCGTTT CTGCGGACTG GCTTTCTACG TAATGGTTTC TTAGACGTCA 2001 GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT 2051 CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA 2101 TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 2151 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA 2201 CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 2251 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT 2301 TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTCA AAGTTCTGCT 2351 ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC 2401 GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 2451 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC 2501 CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACTATCG 2551 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA 2601 ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA 2651 CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC 2701 TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 2751 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC 2801 GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC 2851 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA 2901 GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA 2951 GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA TTCGAAATGA 3001 CCGACCAAGC GACGCCCAAC CGGTATCAGC TCACTCAAAG GCGGTAATAC 3051 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 3101 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 3151 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 3201 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 3251 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 3301 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 3351 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 3401 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 3451 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 3501 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 3551 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG 3601 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 3651 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 3701 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TTTCAAGAAG ATCCTTTGAT 3751 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 3801 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTATAG 3851 TCCGGAAATA CAGGAACGCA CGCTGGATGG CCCTTCGCTG GGATGGTGAA 3901 ACCATGAAAA ATGGCAGCTT CAGTGGATTA AGTGGGGGTA ATGTGGCCTG 3951 TACCCTCTGG TTGCATAGGT ATTCATACGG TTAAAATTTA TCAGGCGCGA 4001 TTGCGGCAGT TTTTCGGGTG GTTTGTTGCC ATTTTTACCT GTCTGCTGCC 4051 GTGATCGCGC TGAACGCGTT TTAGCGGTGC GTACAATTAA GGGATTATGG 4101 TAAATCCACT TACTGTCTGC CCTCGTAGCC ATCGAGATAA ACCGCAGTAC 4151 TCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCT File updated 28-Apr-08.