pFN22K (HaloTag(R)) CMVd1 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN22K (HaloTag(R)) CMVd1 Flexi(R) Vector sequence reference points: Base pairs 4183 CMVd1 intermediate early enhancer/promoter 1-121 T7 RNA polymerase promoter (-17 to +3) 132-151 SP6 RNA polymerase promoter (-17 to +3) 156-175 HaloTag(R) 7 protein coding region 191-1081 TEV region 1094-1114 SgfI region 1121-1128 Barnase coding region 1152-1487 PmeI region 1489-1496 SV40 late polyadenylation signal 1648-1869 Kanamycin resistance (Kanr) coding region 2177-2971 ColE1-derived plasmid origin of replication 3140-3176 cer region (for E. coli XerCD recombinase) 3847-4132 .. 1 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAATAACCCC GCCCCGTTGA 51 CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT 101 GGTTTAGTGA ACCGTCAGAT CACTAGAAGC TTAATACGAC TCACTATAGG 151 GTACGATTTA GGTGACACTA TAGAATAAGG AGAATTCATC ATGGCAGAAA 201 TCGGTACTGG CTTTCCATTC GACCCCCATT ATGTGGAAGT CCTGGGCGAG 251 CGCATGCACT ACGTCGATGT TGGTCCGCGC GATGGCACCC CTGTGCTGTT 301 CCTGCACGGT AACCCGACCT CCTCCTACGT GTGGCGCAAC ATCATCCCGC 351 ATGTTGCACC GACCCATCGC TGCATTGCTC CAGACCTGAT CGGTATGGGC 401 AAATCCGACA AACCAGACCT GGGTTATTTC TTCGACGACC ACGTCCGCTT 451 CATGGATGCC TTCATCGAAG CCCTGGGTCT GGAAGAGGTC GTCCTGGTCA 501 TTCACGACTG GGGCTCCGCT CTGGGTTTCC ACTGGGCCAA GCGCAATCCA 551 GAGCGCGTCA AAGGTATTGC ATTTATGGAG TTCATCCGCC CTATCCCGAC 601 CTGGGACGAA TGGCCAGAAT TTGCCCGCGA GACCTTCCAG GCCTTCCGCA 651 CCACCGACGT CGGCCGCAAG CTGATCATCG ATCAGAACGT TTTTATCGAG 701 GGTACGCTGC CGATGGGTGT CGTCCGCCCG CTGACTGAAG TCGAGATGGA 751 CCATTACCGC GAGCCGTTCC TGAATCCTGT TGACCGCGAG CCACTGTGGC 801 GCTTCCCAAA CGAGCTGCCA ATCGCCGGTG AGCCAGCGAA CATCGTCGCG 851 CTGGTCGAAG AATACATGGA CTGGCTGCAC CAGTCCCCTG TCCCGAAGCT 901 GCTGTTCTGG GGCACCCCAG GCGTTCTGAT CCCACCGGCC GAAGCCGCTC 951 GCCTGGCCAA AAGCCTGCCT AACTGCAAGG CTGTGGACAT CGGCCCGGGT 1001 CTGAATCTGC TGCAAGAAGA CAACCCGGAC CTGATCGGCA GCGAGATCGC 1051 GCGCTGGCTG TCGACGCTCG AGATTTCCGG CGAGCCAACC ACTGAGGATC 1101 TGTACTTTCA GAGCGATAAC GCGATCGCCA TGGAATAAGT AAGGAATCCA 1151 CATGGCACAG GTTATCAACA CGTTTGACGG GGTTGCGGAT TATCTTCAGA 1201 CATATCATAA GCTACCTGAT AATTACATTA CAAAATCAGA AGCACAAGCC 1251 CTCGGCTGGG TGGCATCAAA AGGGAACCTT GCAGACGTCG CTCCGGGGAA 1301 AAGCATCGGC GGAGACATCT TCTCAAACAG GGAAGGCAAA CTCCCGGGCA 1351 AAAGCGGACG AACATGGCGT GAAGCGGATA TTAACTATAC ATCAGGCTTC 1401 AGAAATTCAG ACCGGATTCT TTACTCAAGC GACTGGCTGA TTTACAAAAC 1451 AACGGACCAT TATCAGACCT TTACAAAAAT CAGATAATGT TTAAACGAAT 1501 TCGGGCTCGG TACCCGGGGA TCCTCTAGAG TCGACCTGCA GGCATGCAAG 1551 CTGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC 1601 ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCGGCCG CTTCGAGCAG 1651 ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG 1701 TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT 1751 AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT 1801 TTATGTTTCA GGTTCAGGGG GAGATGTGGG AGGTTTTTTT AAGCAAGTAA 1851 AACCTCTACA AATGTGGTAA AATCGAATTC TAATGGATCC TCTTTGCGCT 1901 TGCGTTTTCC CTTGTCCAGA TAGCCCAGTA GCTGACATTC ATCCGGGGTC 1951 AGCACCGTTT CTGCGGACTG GCTTTCTACG TGTTCCGCTT CCTTTAGCAG 2001 CCCTTGCGCC CTGAGTGCTT GCGGCAGCGT GAGCTTCAAA AGAATTGCCA 2051 GCTGGGGCGC CCTCTGGTAA GGTTGGGAAG CCCTGCAAAG TAAACTGGAT 2101 GGCTTTCTTG CCGCCAAGGA TCTGATGGCG CAGGGGATCA AGATCTGATC 2151 AAGAGACAGG ATGACGGTCG TTTCGCATGC TTGAACAAGA TGGATTGCAC 2201 GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC 2251 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC 2301 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 2351 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT 2401 TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC 2451 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT 2501 CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC 2551 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG 2601 AGCGAGCACG CACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 2651 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA 2701 GGCGCGTATG CCGGATGGTG AGGATCTCGT CGTGACTCAT GGCGATGCCT 2751 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC 2801 TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC 2851 CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG 2901 TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 2951 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC 3001 CAAGCGACGC CCAACCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA 3051 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 3101 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 3151 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG 3201 TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 3251 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 3301 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT 3351 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 3401 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 3451 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT 3501 GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 3551 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT 3601 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA 3651 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 3701 GCAGATTACG CGCAGAAAAA AAGGATTTCA AGAAGATCCT TTGATCTTTT 3751 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 3801 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TATAGTCCGG 3851 AAATACAGGA ACGCACGCTG GATGGCCCTT CGCTGGGATG GTGAAACCAT 3901 GAAAAATGGC AGCTTCAGTG GATTAAGTGG GGGTAATGTG GCCTGTACCC 3951 TCTGGTTGCA TAGGTATTCA TACGGTTAAA ATTTATCAGG CGCGATTGCG 4001 GCAGTTTTTC GGGTGGTTTG TTGCCATTTT TACCTGTCTG CTGCCGTGAT 4051 CGCGCTGAAC GCGTTTTAGC GGTGCGTACA ATTAAGGGAT TATGGTAAAT 4101 CCACTTACTG TCTGCCCTCG TAGCCATCGA GATAAACCGC AGTACTCCGG 4151 CCACGATGCG TCCGGCGTAG AGGATCGAGA TCT File updated 28-Apr-08.