pFN21K (HaloTag(R) 7) CMV Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN21K (HaloTag(R) 7) CMV Flexi(R) Vector sequence reference points: Base pairs 5059 CMV intermediate early enhancer/promoter 1-742 Chimeric intron 857-989 T7 RNA polymerase promoter (-17 to +3) 1033-1052 HaloTag(R) 7 protein coding region 1067-1957 TEV region 1970-1990 SgfI region 1997-2004 Barnase coding region 2028-2363 Pme I region 2365-2372 SV40 late polyadenylation signal 2524-2745 Kanamycin resistance (Kanr) coding region 3035-3847 ColE1-derived plasmid origin of replication 4016-4052 cer region (for E. coli XerCD recombinase) 4723-5008 .. 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCAAA GCCACCATGG CAGAAATCGG TACTGGCTTT CCATTCGACC 1101 CCCATTATGT GGAAGTCCTG GGCGAGCGCA TGCACTACGT CGATGTTGGT 1151 CCGCGCGATG GCACCCCTGT GCTGTTCCTG CACGGTAACC CGACCTCCTC 1201 CTACGTGTGG CGCAACATCA TCCCGCATGT TGCACCGACC CATCGCTGCA 1251 TTGCTCCAGA CCTGATCGGT ATGGGCAAAT CCGACAAACC AGACCTGGGT 1301 TATTTCTTCG ACGACCACGT CCGCTTCATG GATGCCTTCA TCGAAGCCCT 1351 GGGTCTGGAA GAGGTCGTCC TGGTCATTCA CGACTGGGGC TCCGCTCTGG 1401 GTTTCCACTG GGCCAAGCGC AATCCAGAGC GCGTCAAAGG TATTGCATTT 1451 ATGGAGTTCA TCCGCCCTAT CCCGACCTGG GACGAATGGC CAGAATTTGC 1501 CCGCGAGACC TTCCAGGCCT TCCGCACCAC CGACGTCGGC CGCAAGCTGA 1551 TCATCGATCA GAACGTTTTT ATCGAGGGTA CGCTGCCGAT GGGTGTCGTC 1601 CGCCCGCTGA CTGAAGTCGA GATGGACCAT TACCGCGAGC CGTTCCTGAA 1651 TCCTGTTGAC CGCGAGCCAC TGTGGCGCTT CCCAAACGAG CTGCCAATCG 1701 CCGGTGAGCC AGCGAACATC GTCGCGCTGG TCGAAGAATA CATGGACTGG 1751 CTGCACCAGT CCCCTGTCCC GAAGCTGCTG TTCTGGGGCA CCCCAGGCGT 1801 TCTGATCCCA CCGGCCGAAG CCGCTCGCCT GGCCAAAAGC CTGCCTAACT 1851 GCAAGGCTGT GGACATCGGC CCGGGTCTGA ATCTGCTGCA AGAAGACAAC 1901 CCGGACCTGA TCGGCAGCGA GATCGCGCGC TGGCTGTCGA CGCTCGAGAT 1951 TTCCGGCGAG CCAACCACTG AGGATCTGTA CTTTCAGAGC GATAACGCGA 2001 TCGCCATGGA ATAAGTAAGG AATCCACATG GCACAGGTTA TCAACACGTT 2051 TGACGGGGTT GCGGATTATC TTCAGACATA TCATAAGCTA CCTGATAATT 2101 ACATTACAAA ATCAGAAGCA CAAGCCCTCG GCTGGGTGGC ATCAAAAGGG 2151 AACCTTGCAG ACGTCGCTCC GGGGAAAAGC ATCGGCGGAG ACATCTTCTC 2201 AAACAGGGAA GGCAAACTCC CGGGCAAAAG CGGACGAACA TGGCGTGAAG 2251 CGGATATTAA CTATACATCA GGCTTCAGAA ATTCAGACCG GATTCTTTAC 2301 TCAAGCGACT GGCTGATTTA CAAAACAACG GACCATTATC AGACCTTTAC 2351 AAAAATCAGA TAATGTTTAA ACGAATTCGG GCTCGGTACC CGGGGATCCT 2401 CTAGAGTCGA CCTGCAGGCA TGCAAGCTGA TCCGGCTGCT AACAAAGCCC 2451 GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA 2501 CCCCTTGGGG CGGCCGCTTC GAGCAGACAT GATAAGATAC ATTGATGAGT 2551 TTGGACAAAC CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA 2601 ATTTGTGATG CTATTGCTTT ATTTGTAACC ATTATAAGCT GCAATAAACA 2651 AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT CAGGGGGAGA 2701 TGTGGGAGGT TTTTTTAAGC AAGTAAAACC TCTACAAATG TGGTAAAATC 2751 GAATTCTAAT GGATCCTCTT TGCGCTTGCG TTTTCCCTTG TCCAGATAGC 2801 CCAGTAGCTG ACATTCATCC GGGGTCAGCA CCGTTTCTGC GGACTGGCTT 2851 TCTACGTGTT CCGCTTCCTT TAGCAGCCCT TGCGCCCTGA GTGCTTGCGG 2901 CAGCGTGAGC TTCAAAAGAA TTGCCAGCTG GGGCGCCCTC TGGTAAGGTT 2951 GGGAAGCCCT GCAAAGTAAA CTGGATGGCT TTCTTGCCGC CAAGGATCTG 3001 ATGGCGCAGG GGATCAAGAT CTGATCAAGA GACAGGATGA CGGTCGTTTC 3051 GCATGCTTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG 3101 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA 3151 TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA 3201 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG 3251 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT 3301 TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC 3351 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG 3401 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT 3451 CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGCACT CGGATGGAAG 3501 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG 3551 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGTATGCCGG ATGGTGAGGA 3601 TCTCGTCGTG ACTCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA 3651 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC 3701 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG 3751 CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG 3801 ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG 3851 GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCGGTATCAG 3901 CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA 3951 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 4001 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 4051 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA 4101 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 4151 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG 4201 TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC 4251 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 4301 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 4351 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG 4401 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 4451 CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT 4501 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT 4551 AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 4601 ATTTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 4651 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC 4701 TTCACCTAGA TCCTTTTATA GTCCGGAAAT ACAGGAACGC ACGCTGGATG 4751 GCCCTTCGCT GGGATGGTGA AACCATGAAA AATGGCAGCT TCAGTGGATT 4801 AAGTGGGGGT AATGTGGCCT GTACCCTCTG GTTGCATAGG TATTCATACG 4851 GTTAAAATTT ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT GGTTTGTTGC 4901 CATTTTTACC TGTCTGCTGC CGTGATCGCG CTGAACGCGT TTTAGCGGTG 4951 CGTACAATTA AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC 5001 CATCGAGATA AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA 5051 TCGAGATCT File updated 28-Apr-08.