Optimized Protein Purification and Capture with HaloTag® Ligands
HaloTag® Ligands Appropriate for Protein Purification and Pull-Down Assays
- Use cell-permeant biotin ligand for live-cell applications or impermeant PEG-biotin ligand for lysate-based workflows
- Covalently attach affinity tags to membrane or intracellular proteins
- Perform rapid, highly specific labeling that forms a nearly irreversible bond under physiological or recommended conditions
Affinity Tags for Protein Purification
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| G8281 | HaloTag® Biotin Ligand | View Specifications | 30μl | List Price: $ 751.00 Your Price: Log In | Add to Cart | ||
| G8282 | HaloTag® Biotin Ligand | View Specifications | 15μl | List Price: $ 449.00 Your Price: Log In | Add to Cart | ||
| G8591 | HaloTag® PEG-Biotin Ligand | View Specifications | 30μl | List Price: $ 727.00 Your Price: Log In | Add to Cart | ||
| G8592 | HaloTag® PEG-Biotin Ligand | View Specifications | 15μl | List Price: $ 455.00 Your Price: Log In | Add to Cart |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
HaloTag® Ligands
The Promega HaloTag® Ligand portfolio enables a range of functionalities, including affinity labeling for protein purification. These ligands form highly specific and nearly irreversible covalent bonds with HaloTag® fusion proteins under physiological conditions, ensuring stable and efficient labeling with minimal background interference. HaloTag® Ligands enable you to study any compound or surface with a compatible chemical group. With a full suite of HaloTag® Ligand Building Blocks, the possible applications are endless.
HaloTag® Biotin Ligands enable efficient biotin labeling for protein capture and analysis. The HaloTag® Biotin Ligand is cell-permeant, allowing intracellular labeling while unbound ligand can be washed away after incubation.
For enhanced pull-down performance, the HaloTag® PEG-Biotin Ligand offers superior interaction with streptavidin due to its extended and flexible spacer. While it requires cell lysis prior to labeling due to limited membrane permeability, this extended linker can help preserve the activity of HaloTag® fusion proteins during immobilization or derivatization.
Interested in direct capture of HaloTag? Learn about our HaloLink™ Resin.
Gel Analysis of HaloTag-Based Fusion Proteins
Efficient capture of a p65-HaloTag® fusion protein and co-capture of IκB on paramagnetic streptavidin-coated particles. CHO-K1 cells transiently transfected with plasmid encoding the p65-HaloTag® fusion protein were incubated in the presence or absence of 25µM HaloTag® Biotin Ligand. Cells were lysed, and biotin-labeled proteins were captured on Streptavidin-coated MagneSphere® Paramagnetic Particles (Cat.# Z5481). Proteins were resolved by SDS-PAGE and analyzed by Western blot with anti-p65 antibody (BD Bioscience, Panel A) or anti-IκB antibody (BD Bioscience, Panel B). Content of lanes for both Panels A and B: C = cell lysate; UB = unbound protein; B = bound protein.
We offer a suite of HaloTag® Ligands with applications outside of protein purification, including fluorescently tagged proteins for imaging studies.
U2OS cells imaged with XFD 488i HaloTag® Ligand.
Explore the Kazusa Collection, a curated library of HaloTag® human open reading frames (ORFs), clones and panels for streamlined protein expression and functional studies. Leverage this library to expand your experimental possibilities, from affinity purification to imaging applications.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
|
HaloTag® Biotin Ligand |
G828A | 1 × 30μl | 5mM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. RE42931 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
HaloTag® Biotin Ligand |
G828B | 1 × 15μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. RE42931 and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Concentration |
|---|---|---|---|
|
HaloTag® PEG-Biotin Ligand |
G859A | 1 × 30μl | 5mM |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. RE42931 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
HaloTag® PEG-Biotin Ligand |
G859B | 1 × 15μl |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide researcher with a full refund.
Researcher may use this product for research use only; no transfer or commercial use of this product is allowed. Commercial use means any and all uses of this product by a party in exchange for consideration, including, but not limited to (1) use in further product manufacture; (2) use in provision of services, information or data; and (3) resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. With respect to any uses outside this label license, including any commercial, diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
U.S. Pat. No. RE42931 and other patents and patents pending.
Resources
Articles
- Human Flexi® ORF Clones Ready for HaloTag® Labeling
- Expression of Fusion Proteins: How to Get Started with the HaloTag® Technology
- HaloTag® for High Protein Yield and Purity
- Analyzing the Human Proteasome with Proteomics Techniques
- Cell Surface HaloTag® Technology Spatial Separation and Bidirectional Trafficking of Proteins
- HaloTag® Protein: A Novel Reporter Protein for Human Neural Stem Cells
- Simple and Reliable Labeling from Single Wells to High Content Screens
- Image Live and Fixed Cells with Multicolor Interchangeable Fluorescent Labels
- Protein Labeling, Single Well to High-Content Screens
- HaloTag™ Interchangeable Labeling Technology for Cell Imaging Protein Capture and Immobilization
Other Resources
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Frequently Used With
pFC14A and pFC14K HaloTag® CMV Flexi® Vectors
Configured to append the HaloTag to the C-terminus of the protein fusion partner and provide constitutive protein expression in mammalian cells.
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pFN28A and pFN28K HaloTag® CMV-neo Flexi® Vectors
Appends HaloTag® tag to the aminoterminus of the protein fusion partner.
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Streptavidin MagneSphere® Paramagnetic Particles
Allow magnetic separation and purification of biotinylated nucleic acids or proteins.
Z5481, Z5482
ECL Western Blotting Substrate
A sensitive chemiluminescent substrate to detect horseradish peroxidase conjugates on immunoblots. Detect picogram amounts of antigen via photographic or chemiluminescence imaging.
W1001, W1015
