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HaloTag® Flexi® Vectors—CMV Deletion Series Sample Pack

  • Available as a standalone product or as part of the HaloTag® Cloning Starter Kit (Cat.# G6050)

Size

Catalog number selected: G3780

$ 274.00
Your price:
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This product is discontinued
HaloTag® Flexi® Vectors—CMV Deletion Series Sample Pack
9 × 2μg
$ 274.00
Your price: Log in

The vectors contained in the sample pack are configured to append the HaloTag® protein to either the carboxy- (pFC14K, pFC15K, pFC16K and pFC17K vectors) or amino-terminus (pFN21A, pFN21K, pFN22K, pFN23K and pFN24K vectors) of the protein fusion partner and provide constitutive protein expression in mammalian cells. The promoter in this vector series is based on the human cytomegalovirus (CMV) intermediate/early enhancer/promoter to support expression in mammalian cells at physiologically relevant levels. In some cell types, the vector series can provide four levels of expression, ranging from very high (full-length CMV) to very low (CMVd3, the most extreme deletion), spaced about 0.5 to 1 orders of magnitude apart.

Each vector contains the following features:

  • A T7 RNA polymerase promoter for in vitro HaloTag® fusion protein expression. All vectors but pFC14K, pFN21K and pFN21A also have the SP6 RNA polymerase promoter for in vitro expression.
  • A TEV protease site for cleavage of the expressed protein from HaloTag® using ProTEV Protease (Cat.# V6051).
  • The lethal barnase gene for positive selection of the insert.
  • Either an ampicillin-resistance gene (pFN21A) or kanamycin-resistance gene for selection of the plasmid.

 

Specifications

What's in the box?

Item Part # Size

pFC17K HaloTag® CMVd3 Flexi® Vector

G132B 1 × 2μg

pFC16K HaloTag® CMVd2 Flexi® Vector

G157B 1 × 2μg

pFC15K HaloTag® CMVd1 Flexi® Vector

G160B 1 × 2μg

pFN21A HaloTag® CMV Flexi® Vector

G282B 1 × 2μg

pFN21K HaloTag® CMV Flexi® Vector

G283B 1 × 2μg

pFN22K HaloTag® CMVd1 Flexi® Vector

G285B 1 × 2μg

pFN23K HaloTag® CMVd2 Flexi® Vector

G287B 1 × 2μg

pFN24K HaloTag® CMVd3 Flexi® Vector

G298B 1 × 2μg

pFC14K HaloTag® CMV Flexi® Vector

G966B 1 × 2μg

SDS

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Download SDSPDF (237 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund. Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

U.S. Pat. Nos. 8,293,503, 9,018,014 and 8,367,403, European Pat. No. 1685247 and other patents and patents pending.

U.S. Pat. Nos. 7,425,436, 7,935,803, 8,466,269, 8,742,086, 8,420,367, 8,748,148, 9,416,353, 9,593,316 and other patents and patents pending.

Patent Pending.

For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

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