VHL This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 VHL sequence reference points: Base pairs 5246 CMV immediate-early enhancer/promoter 1-742 T7 RNA Polymerase promoter (-17 to +3) 1033-1052 VHL-NanoLuc Fusion Coding Region 1065-2237 Neo-Kan Resistance 3197-3991 ---- 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGAT CGCCATGCCC CGGAGGGCGG AGAACTGGGA CGAGGCCGAG 1101 GTAGGCGCGG AGGAGGCAGG CGTCGAAGAG TACGGCCCTG AAGAAGACGG 1151 CGGGGAGGAG TCGGGCGCCG AGGAGTCCGG CCCGGAAGAG TCCGGCCCGG 1201 AGGAACTGGG CGCCGAGGAG GAGATGGAGG CCGGGCGGCC GCGGCCCGTG 1251 CTGCGCTCGG TGAACTCGCG CGAGCCCTCC CAGGTCATCT TCTGCAATCG 1301 CAGTCCGCGC GTCGTGCTGC CCGTATGGCT CAACTTCGAC GGCGAGCCGC 1351 AGCCCTACCC AACGCTGCCG CCTGGCACGG GCCGCCGCAT CCACAGCTAC 1401 CGAGGTCACC TTTGGCTCTT CAGAGATGCA GGGACACACG ATGGGCTTCT 1451 GGTTAACCAA ACTGAATTAT TTGTGCCATC TCTCAATGTT GACGGACAGC 1501 CTATTTTTGC CAATATCACA CTGCCAGTGT ATACTCTGAA AGAGCGATGC 1551 CTCCAGGTTG TCCGGAGCCT AGTCAAGCCT GAGAATTACA GGAGACTGGA 1601 CATCGTCAGG TCGCTCTACG AAGATCTGGA AGACCACCCA AATGTGCAGA 1651 AAGACCTGGA GCGGCTGACA CAGGAGCGCA TTGCACATCA ACGGATGGGA 1701 GATGTTTCTC TCGGCTCGAG CGGCGTCTTC ACACTCGAAG ATTTCGTTGG 1751 GGACTGGCGA CAGACAGCCG GCTACAACCT GGACCAAGTC CTTGAACAGG 1801 GAGGTGTGTC CAGTTTGTTT CAGAATCTCG GGGTGTCCGT AACTCCGATC 1851 CAAAGGATTG TCCTGAGCGG TGAAAATGGG CTGAAGATCG ACATCCATGT 1901 CATCATCCCG TATGAAGGTC TGAGCGGCGA CCAAATGGGC CAGATCGAAA 1951 AAATTTTTAA GGTGGTGTAC CCTGTGGATG ATCATCACTT TAAGGTGATC 2001 CTGCACTATG GCACACTGGT AATCGACGGG GTTACGCCGA ACATGATCGA 2051 CTATTTCGGA CGGCCGTATG AAGGCATCGC CGTGTTCGAC GGCAAAAAGA 2101 TCACTGTAAC AGGGACCCTG TGGAACGGCA ACAAAATTAT CGACGAGCGC 2151 CTGATCAACC CCGACGGCTC CCTGCTGTTC CGAGTAACCA TCAACGGAGT 2201 GACCGGCTGG CGGCTGTGCG AACGCATTCT GGCGTAAGGC CGCGACTCTA 2251 GAGTCGACCT GCAGGCATGC AAGCTGATCC GGCTGCTAAC AAAGCCCGAA 2301 AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC 2351 CTTGGGGCGG CCGCTTCGAG CAGACATGAT AAGATACATT GATGAGTTTG 2401 GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT 2451 TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT 2501 TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGATGT 2551 GGGAGGTTTT TTTAAGCAAG TAAAACCTCT ACAAATGTGG TAAAATCGAA 2601 TTTTAACAAA ATATTAACGC TTACAATTTC CTGATGCGGT ATTTTCTCCT 2651 TACGCATCTG TGCGGTATTT CACACCGCAT ACGCGGATCT GCGCAGCACC 2701 ATGGCCTGAA ATAACCTCTG AAAGAGGAAC TTGGTTAGGT ACCTTCTGAG 2751 GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC 2801 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC 2851 AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 2901 AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG 2951 CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG 3001 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG 3051 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 3101 AAAAAGCTTA ATTAACTGTT GACAATTAAT CATCGGCATA GTATATCGGC 3151 ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCCAGGAGG CAGATCATGA 3201 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 3251 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC 3301 CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG 3351 ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG 3401 TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC 3451 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC 3501 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 3551 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA 3601 CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC 3651 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC 3701 GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT 3751 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC 3801 GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 3851 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA 3901 ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC 3951 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC 4001 TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGATGG 4051 CCGCAATAAA ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT 4101 GTGAATCGAT AGCGATAAGG ATCCGCTCAT GCGATGATAA GCTGTCAAAC 4151 ATGAGAATTA CAACTTATAT CGTATGGGGC TGACTTCAGG TGCTACATTT 4201 GAAGAGATAA ATTGCACTGA AATCTAGAAA TATTTTATCT GATTAATAAG 4251 ATGATCTTCT TGAGATCGTT TTGGTCTGCG CGTAATCTCT TGCTCTGAAA 4301 ACGAAAAAAC CGCCTTGCAG GGCGGTTTTT CGAAGGTTCT CTGAGCTACC 4351 AACTCTTTGA ACCGAGGTAA CTGGCTTGGA GGAGCGCAGT CACCAAAACT 4401 TGTCCTTTCA GTTTAGCCTT AACCGGCGCA TGACTTCAAG ACTAACTCCT 4451 CTAAATCAAT TACCAGTGGC TGCTGCCAGT GGTGCTTTTG CATGTCTTTC 4501 CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGACT 4551 GAACGGGGGG TTCGTGCATA CAGTCCAGCT TGGAGCGAAC TGCCTACCCG 4601 GAACTGAGTG TCAGGCGTGG AATGAGACAA ACGCGGCCAT AACAGCGGAA 4651 TGACACCGGT AAACCGAAAG GCAGGAACAG GAGAGCGCAC GAGGGAGCCG 4701 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCA 4751 CTGATTTGAG CGTCAGATTT CGTGATGCTT GTCAGGGGGG CGGAGCCTAT 4801 GGAAAAACGG CTTTGCCGCG GCCCTCTCAC TTCCCTGTTA AGTATCTTCC 4851 TGGCATCTTC CAGGAAATCT CCGCCCCGTT CGTAAGCCAT TTCCGCTCGC 4901 CGCAGTCGAA CGACCGAGCG TAGCGAGTCA GTGAGCGAGG AAGCGGAATA 4951 TATCCTGTAT CACATATTCT GCTGACGCAC CGGTGCAGCC TTTTTTCTCC 5001 TGCCACATGA AGCACTTCAC TGACACCCTC ATCAGTGCCA ACATAGTAAG 5051 CCAGTATACA CTCCGCTAGC GCTGATGTCC GGCGGTGCTT TTGCCGTTAC 5101 GCACCACCCC GTCATGAGAT TACGCGTTTT AGCGGTGCGT ACAATTAAGG 5151 GATTATGGTA AATCCACTTA CTGTCTGCCC TCGTAGCCAT CGAGATAAAC 5201 CGCAGTACTC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCT Sequence file prepared 8/21/2020 by Feature database and feature finder version 1.0.5.0.