HaloTag-VHL This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 HaloTag-VHL sequence reference points: Base pairs 5818 CMV promoter 255-845 chimeric intron 960-1092 T7 RNA Polymerase promoter (-17 to +3) 1136-1155 HT7 1170-2060 HaloTag-VHL Coding Region 1170-2753 SgfI Site 2100-2107 PmeI site 2748-2755 Sv40 late polyadenylation signal 2851-3072 EM7 bacterial promoter 3138-3204 Neo-Kan Resistance 3218-4012 ColE1-derived plasmid replication origin 4167-4203 Synthetic PolyAdenylation Signal Sequence 4884-4932 (Reverse) Blasticidin resistance (BlastR) coding region 4956-5354 (Reverse) Sv40 Min Ori 5416-5481 (Reverse) SV40 Enhancer 5488-5724 ---- 1 CGGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT 51 ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA 101 TTCATTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC 151 AAGTAAAACC TCTACAAATG TGGTATGGCT GATTATGATC CGCGGCCGCA 201 ATACGTCGAC GTTATCAGCT GACTTCGTAC GAGAGCCTAG GATTATGGCG 251 CGCCACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC 301 CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC 351 TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC 401 CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT 451 TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT 501 CCGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC 551 CCAGTACATG ACCTTACGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT 601 TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CACCAATGGG 651 CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA 701 CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT 751 GTCGTAATAA CCCCGCCCCG TTGACGCAAA TGGGCGGTAG GCGTGTACGG 801 TGGGAGGTCT ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCACTAG 851 AAGCTTTATT GCGGTAGTTT ATCACAGTTA AATTGCTAAC GCAGTCAGTG 901 CTTCTGACAC AACAGTCTCG AACTTAAGCT GCAGAAGTTG GTCGTGAGGC 951 ACTGGGCAGG TAAGTATCAA GGTTACAAGA CAGGTTTAAG GAGACCAATA 1001 GAAACTGGGC TTGTCGAGAC AGAGAAGACT CTTGCGTTTC TGATAGGCAC 1051 CTATTGGTCT TACTGACATC CACTTTGCCT TTCTCTCCAC AGGTGTCCAC 1101 TCCCAGTTCA ATTACAGCTC TTAAGGCTAG AGTATTAATA CGACTCACTA 1151 TAGGGCTAGC AAAGCCACCA TGGCAGAAAT CGGTACTGGC TTTCCATTCG 1201 ACCCCCATTA TGTGGAAGTC CTGGGCGAGC GCATGCACTA CGTCGATGTT 1251 GGTCCGCGCG ATGGCACCCC TGTGCTGTTC CTGCACGGTA ACCCGACCTC 1301 CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG ACCCATCGCT 1351 GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA ACCAGACCTG 1401 GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT TCATCGAAGC 1451 CCTGGGTCTG GAAGAGGTCG TCCTGGTCAT TCACGACTGG GGCTCCGCTC 1501 TGGGTTTCCA CTGGGCCAAG CGCAATCCAG AGCGCGTCAA AGGTATTGCA 1551 TTTATGGAGT TCATCCGCCC TATCCCGACC TGGGACGAAT GGCCAGAATT 1601 TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC GGCCGCAAGC 1651 TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC GATGGGTGTC 1701 GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG AGCCGTTCCT 1751 GAATCCTGTT GACCGCGAGC CACTGTGGCG CTTCCCAAAC GAGCTGCCAA 1801 TCGCCGGTGA GCCAGCGAAC ATCGTCGCGC TGGTCGAAGA ATACATGGAC 1851 TGGCTGCACC AGTCCCCTGT CCCGAAGCTG CTGTTCTGGG GCACCCCAGG 1901 CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA AGCCTGCCTA 1951 ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT GCAAGAAGAC 2001 AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT CGACGCTCGA 2051 GATTTCCGGC GAGCCAACCA CTGAGGATCT GTACTTTCAG AGCGATAACG 2101 CGATCGCCAT GCCCCGGAGG GCGGAGAACT GGGACGAGGC CGAGGTAGGC 2151 GCGGAGGAGG CAGGCGTCGA AGAGTACGGC CCTGAAGAAG ACGGCGGGGA 2201 GGAGTCGGGC GCCGAGGAGT CCGGCCCGGA AGAGTCCGGC CCGGAGGAAC 2251 TGGGCGCCGA GGAGGAGATG GAGGCCGGGC GGCCGCGGCC CGTGCTGCGC 2301 TCGGTGAACT CGCGCGAGCC CTCCCAGGTC ATCTTCTGCA ATCGCAGTCC 2351 GCGCGTCGTG CTGCCCGTAT GGCTCAACTT CGACGGCGAG CCGCAGCCCT 2401 ACCCAACGCT GCCGCCTGGC ACGGGCCGCC GCATCCACAG CTACCGAGGT 2451 CACCTTTGGC TCTTCAGAGA TGCAGGGACA CACGATGGGC TTCTGGTTAA 2501 CCAAACTGAA TTATTTGTGC CATCTCTCAA TGTTGACGGA CAGCCTATTT 2551 TTGCCAATAT CACACTGCCA GTGTATACTC TGAAAGAGCG ATGCCTCCAG 2601 GTTGTCCGGA GCCTAGTCAA GCCTGAGAAT TACAGGAGAC TGGACATCGT 2651 CAGGTCGCTC TACGAAGATC TGGAAGACCA CCCAAATGTG CAGAAAGACC 2701 TGGAGCGGCT GACACAGGAG CGCATTGCAC ATCAACGGAT GGGAGATGTT 2751 TAAACGAATT CGGGCTCGGT ACCCGGGGAT CCTCTAGAGT CGACCTGCAG 2801 GCATGCAAGC TGATTTGGCT GCTGCCACCG CTGAGCAAGG CCGCTTCGAG 2851 CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG 2901 CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT 2951 TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC 3001 ATTTTATGTT TCAGGTTCAG GGGGAGATGT GGGAGGTTTT TTTAAGCAAG 3051 TAAAACCTCT ACAAATGTGG TAAAATCTTA ATTAAGAATT CTAATATAAC 3101 TTCGTATAGC ATACATTATA CGAAGTTATG GATCCACTGT TGACAATTAA 3151 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA 3201 AACCCAGGAG GCAGATCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 3251 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC 3301 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC 3351 CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG 3401 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 3451 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG 3501 GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 3551 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC 3601 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC 3651 GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG 3701 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT 3751 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA 3801 ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 3851 CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT 3901 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG 3951 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC 4001 GAGTTCTTCT GATTCGAAAT GACCGACCAA GCGACGCCCA ACCGGTATCA 4051 GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 4101 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA 4151 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT 4201 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 4251 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 4301 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC 4351 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 4401 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC 4451 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC 4501 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 4551 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 4601 ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC 4651 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 4701 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG 4751 GATTTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 4801 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 4851 CTTCACCTAG ATCCTTTTAT AGTCCGGAAT CGTCACACAA AAAACCAACA 4901 CACAGATGTA ATGAAAATAA AGATATTTTA TTGCGGCCAT CGTGATGGCT 4951 AGAGTTTAGC CCTCCCACAC ATAACCAGAG GGCAGCAATT CACGAATCCC 5001 AACTGCCGTC GGCTGTCCAT CACTGTCCTT CACTATGGCT TTGATCCCAG 5051 GATGCAGATC GAGAAGCACC TGTCGGCACC GTCCGCAGGG GCTCAAGATG 5101 CCCCTGTTCT CATTTCCGAT CGCGACGATA CAAGTCAGGT TGCCAGCTGC 5151 CGCAGCAGCA GCAGTGCCCA GCACCACGAG TTCTGCACAA GGTCCCCCAG 5201 TAAAATGATA TACATTGACA CCAGTGAAGA TGCGGCCGTC GCTAGAGAGA 5251 GCTGCGCTGG CGACGCTGTA GTCTTCAGAG ATGGGGATGC TGTTGATTGT 5301 AGCCGTTGCT CTTTCAATGA GGGTGGATTC TTCTTGAGAC AAAGGCTTGG 5351 CCATGGTGGC GCTAGTGTCA GAAGAATCGA GCTTTTTGCA AAAGCCTAGG 5401 CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 5451 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG 5501 AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC 5551 GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 5601 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT 5651 GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC 5701 CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGAAGGTACC 5751 TAACCAAGTT CCTCTTTCAG AGGTTATTTC AGGCCATGGT GCTGCGCAAG 5801 ACGCGTTAAT TAAGACGT Sequence file prepared 2/19/2020 by Feature database and feature finder version 1.0.5.0.