We believe this site might serve you best:

United States

United States

Language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Our website does not fully support your browser.

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

BCIP/NBT Color Development Substrate

  • Quality tested and qualified for use in blotting

Size

Catalog number selected: S3771

$ 82.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
BCIP/NBT Color Development Substrate
1.25/2.5ml
$ 82.00
Your price: Log in

BCIP (5-bromo-4-chloro-3-indolyl-phosphate) is used in conjunction with NBT (nitro blue tetrazolium) for the colorimetric detection of alkaline phosphatase activity. Each vial of BCIP is supplied with a vial of NBT.

Preparation of Substrates to Detect Alkaline Phosphatase: For every 5ml of alkaline phosphatase buffer (100mM Tris-HCl [pH 9.0], 150mM NaCl, 1mM MgCl2), add 33μl NBT and 16.5μl BCIP. Add the NBT first, mix, add the BCIP, and mix again. Use within 1 hour, and discard any unused solution.

Concentration: BCIP (50mg/ml) in 100% dimethylformamide; NBT (50mg/ml) in 70% dimethylformamide.

Specifications

What's in the box?

Item Part # Size

NBT

S380C 1 × 2.5ml

BCIP

S381C 1 × 1.25ml

SDS

Choose language:

Download SDSPDF (112 KB) – English (United States)

Certificate of Analysis

See all certificates for S3771

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Let's find the product that meets your needs.

Talk to a Scientist

Alessandro

Alessandro

Italy