We believe this site might serve you best::

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

Real-Time PCR Technologies

 PETAL-Banner-Small

This presentation of real-time polymerase chain reaction (PCR) theory and practice is ideal for nonscientists working in the field of Molecular Biology/Biotechnology or for scientists new to real-time PCR techniques who want to understand the fundamentals and gain laboratory experience.

Dates:
In English: May 26-28, Dec 17-19
In French: April 23-25, Nov 12-14
Additional dates can be arranged by contacting PETAL with a custom training request.

Daily schedule:
9:30 to 17:30 (21 hr)

Fees:
1400 € VAT excluded for researchers and technicians
1200 € VAT excluded for students

Who should attend:
This practical course is for scientists, teachers, laboratory technicians, researchers and others who would like to learn more about real-time PCR, analysis of results, optimizing reactions and troubleshooting problems.

Maximum number of attendees: 12
A certificate of completion is given to participants.

Course Content
This class is a three-day overview of real-time PCR technologies. Course topics include; brief overview of nucleic acid isolation, comparison between end-point and quantitative PCR (qPCR), review of existing qPCR techniques, setting up a SYBR® Green qPCR and multiplexing PCR.

Lectures Laboratory
Nucleic acid isolation fundamentals Isolate and analyze nucleic acids for use in downstream reactions
Comparative review end-point PCR and real-time PCR techniques  
Review of qPCR techniques  
SYBR® Green PCR: Discuss primer design, optimization, data analysis and application to everyday experiments Use SYBR® dye to track amplification in real–time PCR
Multiplexing PCR: Learn how researchers amplify multiple gene products in a single reaction Fluorescent detection of multiple, amplified gene products

Specific course objectives may vary depending on individual and group interests. Please contact us if you have questions.

Register Now!

BTCI-Logo

Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom