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GoTaq® Rapid PCR Master Mix

All-in-One Master Mix to Accelerate Your PCR

  • Fastest PCR master mix available, reducing time-to-results to as little as 15 minutes
  • Ready-to-use mix of optimized components for robust, reliable endpoint PCR
  • Reduce primer-dimer and nonspecific amplification with hot-start technology

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This product is discontinued
GoTaq® Rapid PCR Master Mix

High-Quality, Fast PCR with Results in as Little as 15 Minutes

Optimized for fast and reproducible endpoint PCR assays, the GoTaq® Rapid Master Mix is a ready-to-use PCR master mix designed with you in mind. This master mix speeds your time-to-results without sacrificing result quality or accuracy, so you can tackle high sample volumes and backlogs with ease. 

The GoTaq® Rapid Master Mix includes a Taq polymerase with hot-start antibodies which allow for room-temperature reaction assembly and may reduce primer-dimer and nonspecific DNA amplification. The master mix buffer solution has been optimized to enable the polymerase to combine the annealing and extension phases of a traditional PCR.

Where traditional PCR master mixes can take 1–2 hours to amplify samples, the GoTaq® Rapid Master Mix can decrease that time to as little as 15 minutes, depending on your instrument and target amplicon size. When paired with an instrument with a rapid temperature ramp rate, like the Eppendorf Mastercycler® X50s, amplicons 1kb or greater can be amplified in approximately 15 minutes using the GoTaq® Rapid Master Mix.

Fast PCR illustration for endpoint pcr 17540ma

GoTaq® Rapid PCR Master Mix is Suitable for Different Organisms and Sample Types

The data presented below illustrate the performance of GoTaq® Rapid Master Mix in amplification of human genomic DNA (gDNA), C. albicans and E. coli samples.

Human beta-2-microglobulin

Fast PCR amplification of human genomic DNA with GoTaq Rapid PCR Master Mix.

C. albicans KER1 gene

Fast PCR amplification of C. albicans DNA with GoTaq Rapid PCR Master Mix.

E. coli 16S ribosomal primers

Performance of GoTaq Rapid PCR Master Mix with E. coli 16S ribosomal primers.

*Actual Amplicon Lengths (bp)

Amplicon Fungal Bacteria
200 205 203
400 391 409
600 598 601
800 793 792
1000 997 1021
1200 1195 1196
1400 1403 1398
1600 1605 1600

Cycling Settings

Description Cycles Time Temperature
Activation 1 1 min 94°C
Denaturation 35 2 sec 95°C
Annealing/
Extension
6 sec 65°C
Elongation 1 1 min 72°C
Endpoint PCR amplification from samples with GoTaq® Rapid Master Mix. DNA (50ng) from human genomic DNA (gDNA), C. albicans and E. coli was amplified using the Eppendorf Mastercycler® X50s and 13 minute total cycling time. GoTaq® Rapid Master Mix was able to amplify a 1kb amplicon from all three sample types in under 15 minutes.

Fast PCR That Doesn't Compromise Quality

For human genomic DNA (gDNA), researchers can adjust their extension phase based on their desired target amplicon length. Typically, as the extension cycle time increases, the clarity of bands of a given length improves. However, this extension time analysis highlights the ability of the GoTaq® Rapid Master Mix to produce clear bands of increasingly longer targets in just a 15 minute cycling time. Bacterial and fungal DNA samples also experience improved banding under similar conditions and would amplify longer targets (1kb or greater) in the same 15 minute time frame (data not shown).
As PCR extension cycle time increases, the clarity of bands of a given length improves.
Extension Time Total Cycling Time for
35 Cycles (min)
2 11
4 12
6 13
8 14
10 15
GoTaq® Rapid Master Mix produces clear bands of increasingly longer targets in a 15 minute cycling time.

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Elisa

Elisa

Spain