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GoTaq® MDx Hot Start Polymerase

Inhibits Polymerase Activity at Temperatures below 70°C

  • Manufactured under cGMP
  • High-concentration and glycerol-free formulations also available
  • All formulations supplied at ≥5u/μl, except for high-concentration formulation, which is supplied at ≥50u/μl
  • Custom formats and formulations

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Catalog number selected: D6001

$ 81.00
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GoTaq® MDx Hot Start Polymerase
Hot Start Polymerase/100u
$ 81.00
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34605099-OverviewImage

Consistent and Robust Amplification

GoTaq® MDx Hot Start Polymerase contains GoTaq® MDx DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 94–95°C for two minutes. This allows hot-start PCR in which polymerase activity is inhibited at temperatures below 70°C, allowing convenient, room-temperature reaction setup. Hot-start PCR is advantageous for some amplification targets because primer-dimer and secondary products are eliminated or minimized. In some cases, hot-start PCR may improve yields. The glycerol-free product formulation is further purified to remove glycerol, making the enzyme suitable for further manufacture processing and lyophilization.

  • Manufactured under cGMP using validated equipment, processes and method under a certified Quality System to ensure consistent product performance lot-to-lot.
  • QC-tested for bacterial, fungal and mammalian DNA to ensure low DNA contamination.
  • Flexible product formats and custom formulations available.

GoTaq® MDx Hot Start Polymerase is a general purpose reagent intended for general laboratory use. It is suitable for use as a component of molecular diagnostic assays, where applicable country laws allow. Separate license or royalty payments not required. This product alone does not provide any diagnostic result.

PCR Satisfaction Guarantee

Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account.

That's Our PCR Guarantee!

Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.

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Consistent Performance

Variance of 12 separately manufactured lots of product from manufacture target concentration

GoTaq® MDx DNA Polymerase products are manufactured under cGMP using validated equipment, processes and methods under a certified Quality System to ensure consistent product performance lot-to-lot.

Reduce nonspecific amplification with hot-start PCR using GoTaq® MDx Hot Start Polymerase

High Quality

GoTaq® MDx DNA Polymerase products meet stringent specifications for:
Purity ≥90% (Reverse Phase- HPLC)
DNA Contamination One unit contains less than:
  • 10 copies of bacterial genomic DNA
  • 1 genome equivalent of fungal genomic DNA
  • 1 genome equivalent of mammalian genomic DNA
Nuclease Contamination Undetectable exonuclease, endonuclease, nicking and ribonuclease activity
Performance Quantitative amplification of DNA target. Amplification of hot-start model template produces a single 1.5kb band without extraneous bands (GoTaq® MDx Hot Start Polymerase formulation only) 
Stability Stable for 24 months from date of manufacture

Customization

Rely on an Expert Partner

Choose a trusted partner with the robust and reliable reagents you need for your molecular assay and the experience to support you throughout your assay development process.

More than 30 years of manufacturing experience

Commitment to high-quality standards

  • ISO 13485 & ISO 9001 certified
  • cGMP-manufacturing capabilities

Consistent and reliable amplification products

Flexible, custom, made-to-order solutions available

To use GoTaq® MDx Hot Start Polymerase in a custom format or to distribute GoTaq® MDx Hot Start Polymerase, contact the Promega Custom Order Department to discuss specific requirements.

34605099-CustomizationGraphic

Specifications

You are viewing: D6001 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 1 × 750μl25mM View Product

GoTaq® MDx Hot Start Polymerase

D600A 1 × 100u

5X Colorless GoTaq® Flexi Buffer

M890A 1 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 1 × 1ml View Product

SDS

Choose language:

Download SDSPDF (117 KB) – English (United States)

Certificate of Analysis

See all certificates for D6001

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Specifications

You are viewing: D6005 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 3 × 750μl25mM View Product

GoTaq® MDx Hot Start Polymerase

D600B 1 × 500u

5X Colorless GoTaq® Flexi Buffer

M890A 4 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 4 × 1ml View Product

SDS

Choose language:

Download SDSPDF (117 KB) – English (United States)

Certificate of Analysis

See all certificates for D6005

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Specifications

You are viewing: D6006 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 15 × 750μl25mM View Product

GoTaq® MDx Hot Start Polymerase

D600B 5 × 500u

5X Colorless GoTaq® Flexi Buffer

M890A 20 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 20 × 1ml View Product

SDS

Choose language:

Download SDSPDF (117 KB) – English (United States)

Certificate of Analysis

See all certificates for D6006

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Specifications

You are viewing: D6008 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 60 × 750μl25mM View Product

GoTaq® MDx Hot Start Polymerase

D600B 20 × 500u

5X Colorless GoTaq® Flexi Buffer

M890A 80 × 1ml View Product

5X Green GoTaq® Flexi Buffer

M891A 80 × 1ml View Product

SDS

Choose language:

Download SDSPDF (117 KB) – English (United States)

Certificate of Analysis

See all certificates for D6008

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Specifications

You are viewing: D6101 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351H 2 × 1.2ml25mM View Product

GoTaq® MDx Hot Start Polymerase

D610A 1 × 1,000u

5X Colorless GoTaq® Flexi Buffer

M890C 2 × 5ml View Product

SDS

Choose language:

Download SDSPDF (91 KB) – English (United States)

Certificate of Analysis

See all certificates for D6101

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

Specifications

You are viewing: D6201 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 3 × 750μl25mM View Product

GoTaq® MDx Hot Start Polymerase, Glycerol-Free

D620A 1 × 500u

5X Colorless GoTaq® Flexi Buffer

M890A 4 × 1ml View Product

SDS

Choose language:

Download SDSPDF (91 KB) – English (United States)

Certificate of Analysis

See all certificates for D6201

Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

BB

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