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Universal RiboClone™ cDNA Synthesis System

C4360_Universal-Riboclone
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  • Reagents to synthesize double-stranded cDNA from up to 40µg of mRNA
  • Oligo(dT)15 Primer and Random Primers included for choice of priming methods
  • Materials provided for 10 adaptor ligation reactions

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Catalog number selected: C4360

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Universal RiboClone™ cDNA Synthesis System
1 system
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The Universal RiboClone™ cDNA Synthesis System is used for double-stranded cDNA synthesis from RNA. The system also includes materials for ligation of the cDNA into a suitable vector. First-strand synthesis is driven by Avian Myeloblastosis Virus (AMV) Reverse Transcriptase and either Random Primers or Oligo(dT)15 Primer. This is followed directly by second-strand synthesis using RNase H and DNA Polymerase I. After treatment with T4 DNA Polymerase to create blunt ends, the double-stranded cDNA molecules are prepared for cloning by size fractionation and addition of EcoRI Adaptors. The resulting cDNA preparation is ready for cloning into a suitable vector.

Protocols

Complete Protocol

Specifications

What's in the box?

Item Part # Size Concentration Available Separately

Sodium Pyrophosphate

 C113A 1 × 50μl 40mM

First Strand 5X Buffer

 C121A 1 × 200μl

T4 DNA Ligase 10X Buffer

 C126A 1 × 500μl View Product

Spin Columns

 C1281 1 × 10 each View Product

EcoRI Adaptors

 C129A 1 × 150pmol View Product

Kinase 10X Buffer

 C131A 1 × 500μl View Product

ATP

 C134A 1 × 50μl

1.2kb Kanamycin Positive Control RNA

 C138B 1 × 10μg 0.5mg/ml View Product

Oligo(dT)15 Primer

 C144A 1 × 20μg 500μg/ml View Product

Random Primers

 C146A 1 × 20μg View Product

Second Strand 2.5X Buffer, AMV

 C630A 1 × 800μl

T4 DNA Ligase

 M180A 1 × 100u

DNA Polymerase I

 M205A 1 × 500u

T4 Polynucleotide Kinase

 M410A 1 × 100u View Product

T4 DNA Polymerase

 M421A 1 × 100u

RNase H

 M428B 1 × 20u

AMV Reverse Transcriptase (HC)

 M900A 1 × 600u View Product

Recombinant RNasin® Ribonuclease Inhibitor

 N251E 1 × 1,000u

Nuclease-Free Water

 P119A 1 × 1,250μl View Product

Acetylated BSA

 R946A 1 × 400μl View Product

Sephacryl® S-400

 V318A 1 × 10ml View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

Resources

Citations

Similar Products

M-MLV Reverse Transcriptase

RNA-dependent DNA polymerase for cDNA synthesis.

M1701, M1705, M5313

Frequently Used With

Oligo(dT)15 Primer

Primer for first-strand cDNA synthesis of mRNAs.

C1101

Random Primers

Random hexamers for universal cDNA synthesis.

C1181

EcoRI Adaptors

Complementary oligonucleotides to add EcoRI sticky ends to blunt-ended DNA.

C1291

1.2kb Kanamycin Positive Control RNA

Supplied in 10mM Tris-HCl (pH 7.4), 1mM EDTA.

C1381

AMV Reverse Transcriptase

Reverse transcriptase with stability at higher reaction temperatures.

M5101, M5108, M9004

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