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Universal RiboClone™ cDNA Synthesis System

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Universal RiboClone™ cDNA Synthesis System
The Universal RiboClone™ cDNA Synthesis System is used for double-stranded cDNA synthesis from RNA. The system also includes materials for ligation of the cDNA into a suitable vector. First-strand synthesis is driven by Avian Myeloblastosis Virus (AMV) Reverse Transcriptase and either Random Primers or Oligo(dT)15 Primer. This is followed directly by second-strand synthesis using RNase H and DNA Polymerase I. After treatment with T4 DNA Polymerase to create blunt ends, the double-stranded cDNA molecules are prepared for cloning by size fractionation and addition of EcoRI Adaptors. The resulting cDNA preparation is ready for cloning into a suitable vector.


What's in the box?

Item Part # Size Concentration Available Separately

Sodium Pyrophosphate

C113A 1 × 50μl 40mM

First Strand 5X Buffer

C121A 1 × 200μl

T4 DNA Ligase 10X Buffer

C126A 1 × 500μl View Product

Spin Columns

C1281 1 × 10 each View Product

EcoRI Adaptors

C129A 1 × 150pmol View Product

Kinase 10X Buffer

C131A 1 × 500μl View Product


C134A 1 × 50μl

1.2kb Kanamycin Positive Control RNA

C138B 1 × 10μg 0.5mg/ml View Product

Oligo(dT)15 Primer

C144A 1 × 20μg 500μg/ml View Product

Random Primers

C146A 1 × 20μg View Product

Second Strand 2.5X Buffer, AMV

C630A 1 × 800μl

T4 DNA Ligase

M180A 1 × 100u

DNA Polymerase I

M205A 1 × 500u

T4 Polynucleotide Kinase

M410A 1 × 100u View Product

T4 DNA Polymerase

M421A 1 × 100u

RNase H

M428B 1 × 20u

AMV Reverse Transcriptase (HC)

M900A 1 × 600u View Product

Recombinant RNasin® Ribonuclease Inhibitor

N251E 1 × 1,000u

Nuclease-Free Water

P119A 1 × 1,250μl View Product

Acetylated BSA

R946A 1 × 400μl View Product

Sephacryl® S-400

V318A 1 × 10ml View Product

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Please see the Protocol for detailed storage conditions.

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