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Tth DNA Polymerase

R6011
PCR Satisfaction Guaranteed

Convenient First-Strand and Second-Strand cDNA Synthesis

  • Catalyzes DNA polymerization at 74°C
  • Uses DNA or RNA as a template, depending on the divalent cation present

Size

Catalog number selected: M2101

$ 94.00 Your price: Log In

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Tth DNA Polymerase
100u
$ 94.00
Your price: Log In
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Tth DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus thermophilus HB-8. The enzyme replicates DNA at 74°C and exhibits a half-life of 20 minutes at 95°C. Tth DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and the polymerization of nucleotides into DNA using an RNA template in the 5´→3´ direction in the presence of manganese. The enzyme also possesses a 5´→3´ exonuclease activity. Tth DNA Polymerase is recommended for use in PCR, RT-PCR, reverse transcription and primer extension reactions at elevated temperatures.

Components

10X Reverse Transcription Buffer: 100mM Tris-HCl (pH 8.3 at 25°C), 900mM KCl.

10X Chelate Buffer: 100mM Tris-HCl (pH 8.3 at 25°C), 1M KCl, 7.5mM EGTA, 0.5% Tween® 20, 50% glycerol.

Thermophilic DNA Polymerase 10X Reaction Buffer: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25°C) and 1% Triton® X-100. Buffer is optimized for use with 0.2mM of each of the dNTPs.

Manganese and Magnesium Chloride: 10mM MnCl2 and 25mM MgCl2 Solutions provided.

Advantages

  • Increased Specificity for RT-PCR: The ability to reverse transcribe at higher temperatures results in increased specificity of primer hybridization and extension.
  • Minimized Secondary Structures: Higher-temperature RT-PCR minimizes problems associated with strong secondary structures in RNA.
  • Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

Use Tfl polymerase for:

  • RT-PCR.
  • PCR.
  • Primer extension.
  • Reverse transcription.
  1. Ruttimann, C. et al. (1985) Eur. J. Biochem. 149, 41–6.

Protocols

Specifications

You are viewing: 100u Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 1 x 750μl25mM View Product
Thermophilic DNA Polymerase 10X Buffer M190A 1 x 750μl

Tth DNA Polymerase

M210A 1 x 100u

MnCl2

M212A 1 x 750μl10mM

RT 10X Buffer

M213A 1 x 750μl

Chelate 10X Buffer

M214A 1 x 750μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by applicable US patents and corresponding patent claims outside the US held by Roche. The purchase of this product includes a limited, non-transferable immunity from suit under such patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Additional rights for use in non-diagnostic fields require a license from Life Technologies. Diagnostic uses require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting Out-licensing at Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad California 92008; outlicensing@lifetech.com.

Specifications

You are viewing: 500u Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 1 x 750μl25mM View Product
Thermophilic DNA Polymerase 10X Buffer M190A 1 x 750μl

Tth DNA Polymerase

M210B 1 x 500u

MnCl2

M212A 1 x 750μl10mM

RT 10X Buffer

M213A 1 x 750μl

Chelate 10X Buffer

M214A 1 x 750μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

NOTICE TO PURCHASER: LIMITED LICENSE
Use of this product is covered by applicable US patents and corresponding patent claims outside the US held by Roche. The purchase of this product includes a limited, non-transferable immunity from suit under such patent claims for using only this amount of product for the purchaser’s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Additional rights for use in non-diagnostic fields require a license from Life Technologies. Diagnostic uses require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting Out-licensing at Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad California 92008; outlicensing@lifetech.com.

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