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Plexor® qPCR and RT-qPCR Systems

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Plexor® qPCR and RT-qPCR Systems

Multiplex Real-Time PCR

The Plexor® qPCR and RT-qPCR Systems are multiplex-capable real-time amplification systems using novel base pair chemistry. Each target is measured directly during the amplification process and not through a secondary reaction. Plexor® reactions require only two primers for each target.

The Plexor® Systems work by measuring a reduction in fluorescent signal during amplification. Amplification uses only two primers, one of which contains both a fluorescent tag and modified base. As amplification proceeds, fluorescence is reduced by site-specific incorporation of a fluorescent quencher inserted opposite the complementary modified base. The quencher is in close proximity to a fluorescent dye located on the end of the primer, resulting in a reduction in fluorescent signal. After PCR, a melt analysis can be performed to provide an internal control for the final assay design or to expedite troubleshooting during development. The system also includes a proprietary reagent to minimize primer-dimer formation.

Advantages
  • Simplify Multiplex Performance: Only two primers are required for each target, making the design of multiplex assays much easier.
  • Improve Productivity: Less labor, less time and less cost per assay. Measure controls and targets at the same time in the same well.
  • Enjoy Convenience: The master mix format provides everything you need in one tube. Combine with your template and primers.
  • Obtain Strong Data: Plexor® Systems measure a reduction in fluorescent signal during amplification. Quenching is directly proportional to amplicon accumulation. After amplification, a melt analysis can be performed to provide an internal control for specificity.
  • Use Your Existing Real-Time Instruments: Plexor® technology works on most currently available real-time instruments capable of measuring more than one fluor. The free Plexor® Analysis Software allows users to import and analyze data from their preferred instrument platform. For an up-to-date list of supported instruments, visit the Plexor® Resources page.
  • Three Simple Steps to Use Plexor® Systems:
    • Step 1. Design Your Assay: Choose your primer sets, then order from your preferred oligo provider.
    • Step 2. Run the Assay: Instruction manuals are available for a wide variety of real-time instruments including those from Applied Biosystems and Roche.
    • Step 3. Analyze Your Data: Export the raw data from your real-time instrument, then import into the free Plexor® Analysis Software. The Plexor® software converts the quenching data into cycle threshold (Ct) values and generates standard curves.
Applications
  • RT-qPCR.
  • qPCR.
  • SNP genotyping.

Further Reading

Visit the Plexor® Resources page to learn more.

  1. Johnson, S.C. et al. (2004) Nucl. Acids Res. 32, 1937–41.
  2. Frackman, S. et al. (2005) Promega Notes 90, 2–4.
  3. Sherrill, C.B. et al. (2004) J. Am. Chem. Soc. 126, 4550–6.
  4. Moser, M.J. et al. (2005) Antimicrob. Agents Chemother. 49, 3334–40.

Specifications

You are viewing: A4011 Change Configuration

What's in the box?

Item Part # SizeAvailable Separately

Plexor® Master Mix, 2X

A403A 2 × 1.25ml

Nuclease-Free Water

P119A 2 × 1,250μl View Product

MOPS/EDTA Buffer

Y510A 3 × 10ml

Plexor® qPCR Control, 5X

Y523A 1 × 25μl

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: A4021 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

Plexor® Master Mix, 2X

A403A 2 × 1.25ml

1.2kb Kanamycin Positive Control RNA

C138A 1 × 5μg0.5mg/ml View Product

ImProm-II™ Reverse Transcriptase

M314B 2 × 10μl

ImProm-II™ Enzyme Diluent

M409A 1 × 50μl

RNasin® Plus RNase Inhibitor

N261A 2 × 2,500u40u/μl

Nuclease-Free Water

P119A 2 × 1,250μl View Product

MOPS/EDTA Buffer

Y510A 3 × 10ml

Plexor® Kanamycin Control Primer Pair, 25X

Y522A 1 × 10μl

SDS

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Certificate of Analysis

Search for Specific Certificate:

View more results
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Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: A4051 Change Configuration

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 1 × 750μl25mM View Product

Plexor® Master Mix, 2X

A403A 2 × 1.25ml

Oligo(dT)15 Primer

C110A 1 × 20μg500μg/ml View Product

dNTP Mix

C114A 1 × 50μl10mM

Random Primers

C118A 1 × 20μg View Product

1.2kb Kanamycin Positive Control RNA

C138A 1 × 5μg0.5mg/ml View Product

ImProm-II™ 5X Reaction Buffer

M289B 1 × 100μl

ImProm-II™ Reverse Transcriptase

M314B 1 × 10μl

RNasin® Plus RNase Inhibitor

N261A 1 × 2,500u40u/μl

Nuclease-Free Water

P119A 2 × 1,250μl View Product

MOPS/EDTA Buffer

Y510A 3 × 10ml

Plexor® Kanamycin Control Primer Pair, 25X

Y522A 1 × 10μl

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
View more results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB
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