We believe this site might serve you best:

United States

United States

Choose language: English

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

GoTaq® 2-Step RT-qPCR System for Dye-Based Detection

A6010
PCR Satisfaction Guaranteed

Optimized Components for Robust RNA Expression Analysis

  • Integrated reagents for reverse transcription and downstream qPCR
  • Compatible with both fast and standard cycling methods
  • Resistant to a wide range of PCR inhibitors

Size

Catalog number selected: A6010

$ 498.00 Your price: Log In

Add to Helix
GoTaq® 2-Step RT-qPCR System for Dye-Based Detection
5ml
$ 498.00
Your price: Log In

Reliable, Dye-Based Detection of RNA Expression Levels

The GoTaq® 2-Step RT-qPCR System facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components: GoScript™ Reverse Transcription System and GoTaq® qPCR Master Mix.

The master mix is provided as a ready-to-use, stabilized 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs, BRYT Green® Dye and a proprietary reaction buffer. This master mix contains a low level of carboxy-X-rhodamine (CXR); a separate tube of CXR reference dye is included.

The GoScript™ Reverse Transcription System includes an optimized reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

Advantages of the GoTaq® 2-Step RT-qPCR System:

  • Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
  • Compatible with both fast and standard cycling methods on most real-time PCR instruments.
  • Exceptional room-temperature setup is easy for automated and high-throughput detection.

Cat.# A6010 contains sufficient reagents for 50 × 20µl RT reactions and 200 × 50µl qPCR reactions; 50 × 20µl RT reactions and 500 × 20µl qPCR reactions; or 50 × 20µl RT reactions and 1,000 × 10µl qPCR reactions.

PCR Satisfaction Guarantee
Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account.

That's Our PCR Guarantee!

Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.

Protocols

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 1 × 750μl25mM View Product

GoScript™ 5X Reaction Buffer

A500B 1 × 300μl View Product

GoScript™ Reverse Transcriptase

A501B 1 × 50μl

GoTaq® qPCR Master Mix, 2X

A600A 5 × 1ml View Product

Oligo(dT)15 Primer

C110B 1 × 50μg500μg/ml View Product

PCR Nucleotide Mix

C114G 1 × 200μl10mM

Random Primers

C118B 1 × 50μg View Product

CXR Reference Dye

C541A 1 × 100μl View Product

Recombinant RNasin® Ribonuclease Inhibitor

N251A 1 × 2,500u40u/μl

Nuclease-Free Water

P119E 2 × 13ml View Product

SDS

Choose language:

Certificate of Analysis

Search for Specific Certificate:

View more results
No results
Loading…

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

Resources

workflow-background

RNA Analysis Workflow

Choose your country

Americas

Brazil
Brazil
Canada
Canada
United States
United States

Pacific Asia

Australia
Australia
India
India
Japan
Japan
Korea, Republic of
Korea, Republic of
Singapore
Singapore

Europe

Austria
Austria
Belgium
Belgium
Denmark
Denmark
Estonia
Estonia
Finland
Finland
France
France
Germany
Germany
Iceland
Iceland
Italy
Italy
Luxembourg
Luxembourg
Netherlands
Netherlands
Norway
Norway
Poland
Poland
Spain
Spain
Sweden
Sweden
Switzerland
Switzerland
United Kingdom
United Kingdom