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GoTaq® 1-Step RT-qPCR System for Dye-Based Detection

A6020
PCR Satisfaction Guaranteed

RNA Expression Analysis in a Single Reaction

  • One-step RT-qPCR, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix
  • Compatible with both fast and standard cycling methods
  • Resistant to a wide range of PCR inhibitors

Size

Catalog number selected: A6020

$ 575.00 Your price: Log in

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GoTaq® 1-Step RT-qPCR System for Dye-Based Detection
5ml
$ 575.00
Your price: Log in

Relative Quantification of RNA Expression in a Single Reaction

The GoTaq® 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR Master Mix in a single-step real-time amplification reaction.

The GoScript™ RT Mix for 1-Step RT-qPCR (50X) combines optimized quantities amounts of GoScript™ Reverse Transcriptase, RNasin® Plus RNase Inhibitor and additives to enhance single-step reactions.

  • Achieve Superior Performance: Sensitive detection for earlier quantitation of low- and high-copy-number targets over a broad dynamic range.
  • Rely on a Robust System: Resistant to a wide range of PCR inhibitors.
  • Use a Versatile Product: Compatible with both fast and standard cycling methods on most real-time PCR instruments.
  • Realize Enhanced Stability: Exceptional room-temperature setup is easy for automated and high-throughput detection.

PCR Satisfaction Guarantee

Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure reliable, high performance results. Your success is important to us. Our products are backed by a worldwide team of Technical Support scientists. Please contact them for applications or technical assistance. If you are not completely satisfied with any Promega PCR product we will send a replacement or refund your account.

That's Our PCR Guarantee!
Product must be within expiration date and have been stored and used in accordance with product literature. See Promega Product Insert for specific tests performed.

13247MA.eps
Expression levels for the GRAS gene in oil palm seeds were evaluated via 1-step RT-qPCR using the GoTaq® 1-Step RT-qPCR System (Cat. # A6020).

Specifications

What's in the box?

Item Part # SizeConcentrationAvailable Separately

MgCl2

A351B 1 × 750μl25mM View Product

GoTaq® qPCR Master Mix, 2X

A600A 5 × 1ml View Product

Nuclease-Free Water

P119E 2 × 13ml View Product

CXR Reference Dye

C541B 1 × 200μl30μM View Product

GoScript™ RT Mix for 1-Step RT-qPCR

M700A 1 × 225μl50X

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.

U.S. Pat. Nos. 8,598,198 and 9,206,474 and other patents and patents pending.

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