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Tfl DNA Polymerase

DNA Synthesis at Elevated Temperatures

  • Provided with Mg-free 10X Reaction Buffer and separate 25mM MgSO4 for optimization
  • Works with DNA or RNA templates, dependent on the presence of different divalent cations
  • Useful for PCR, 3´ A-tailing of blunt ends and primer extension reactions
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Size

Catalog number selected: M1941

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Tfl DNA Polymerase
100u
$ 53.00
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Tfl DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus flavus. The enzyme replicates DNA at 74°C and exhibits a half-life of 40 minutes at 95°C. Tfl DNA Polymerase catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium and the polymerization of nucleotides into DNA using an RNA template in the 5´→3´ direction in the presence of manganese. The enzyme also possesses a 5´→3´ exonuclease activity. Tfl DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures.

Tfl DNA Polymerase is provided with a 10X Reaction Buffer that does not contain magnesium. Sufficient 25mM MgSO4 is provided separately to allow optimization of enzyme performance under different conditions.

  • 10X Reaction Buffer: 200mM Tris-acetate (pH 8.9 at 25°C), 100mM ammonium sulfate.
  • Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10nmol of dNTPs into TCA-insoluble material in 30 minutes at 74°C.
  • Source: Thermus flavus.
  • Storage Buffer: 20mM Tris-HCl (pH 8.0 at 25°C), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Nonidet®-P40, 0.5% Tween® 20 and 50% glycerol.

Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.

  1. Kaledin, A.S. et al. (1981) Biokhimiia 46, 1576–84.

Specifications

You are viewing: 100u Change Configuration

What's in the box?

Item Part # SizeConcentration

Tfl DNA Polymerase

M194A 1 x 100u

MgSO4

M197A 1 x 1,250μl25mM

Tfl 10X Reaction Buffer

M200A 1 x 750μl

SDS

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

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Specifications

You are viewing: 1,000u Change Configuration

What's in the box?

Item Part # SizeConcentration

Tfl DNA Polymerase

M194B 1 x 1,000u

MgSO4

M197A 4 x 1,250μl25mM

Tfl 10X Reaction Buffer

M200B 5 x 1.2ml

SDS

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Certificate of Analysis

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Use Restrictions

For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

Storage Conditions

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