The proven robust amplification using GoTaq® Polymerase is now available for long-range PCR (up to 30kb gDNA).
GoTaq® Long PCR Master Mix contains the high-performance GoTaq® Hot Start Polymerase in a specially formulated mixture with a proprietary thermal stable proofreading polymerase. This optimized enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. The presence of a proofreading enzyme to repair DNA mismatches and a highly processive polymerase allows the polymerase to continue to elongate the DNA much further, resulting in longer DNA amplification. The optimized formulation of the GoTaq® Long PCR Master Mix components enables simple reaction setup, provides consistently accurate and robust amplification of long DNA amplicons, and is ideal for cloning genes, mutational analysis and DNA sequencing.
GoTaq® Long PCR Master Mix is supplied with a control primer pair and human genomic DNA template to perform control reactions and test template quality.