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PureYield™ RNA Midiprep System Technical Manual

Instructions for Use of Product(s)
Z3740, Z3741

Literature # TM279

The PureYield™ RNA Midiprep System is designed to quickly and easily isolate high yields of pure total RNA from essentially any sample type and virtually eliminates the copurification of DNA. The PureYield™ Clearing Agent preferentially binds DNA, leaving the RNA virtually free of DNA. The streamlined, universal protocol uses the Promega PureYield™ silica-membrane technology to isolate intact RNA, ranging in size from less than 200 bases to greater than 20kb. High-quality total RNA is isolated with minimal effort for a wide range of applications.

Summary of Changes
The following changes were made to the 8/16 revision of this document:
1. The amount and concentration of the β-Mercaptoethanol component was changed, Sections 2 and 3.C.
2. The document design was updated.

Printed in USA. Revised 8/16.

Experienced User Protocols