Wizard® Genomic DNA Purification Kit

Human whole blood

Yes

Yes

Protocols are provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050 for samples of up to 10ml blood or in 96-well plates ("Isolating Genomic DNA from Whole Blood" sections). The protocol works with the following anticoagulants: EDTA, heparin and citrate.
Reference:
Rizzo, W.B., Carney, G. and Lin, Z. (1999) Am. J. Hum. Genet. 65, 1547–60. PubMed ID #10577908

Mouse whole blood

Yes

A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050.

Human peripheral blood mononuclear cells

Yes

Tan, A. et al. (2006) Clin. Chem. 52, 2250–7.
PubMed ID #17040960

Bovine whole blood

Yes

Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348

Horse whole blood

Yes

Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348

Sheep whole blood

Yes

Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348

Antelope whole blood

Yes

Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348

Dog whole blood

Yes

Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348

Cat whole blood

Yes

Walker, J.A. et al.(2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348

Rabbit whole blood

Yes

Walker, J.A. et al. (2003) Anal. Biochem. 316, 259–69.
PubMed ID #12711348

Mouse liver

Yes

Yes

Tissue needs to be homogenized prior to isolation. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section.
Reference:
Barazzoni, R. et al. (2005) Endocrinology 146, 2098–106.
PubMed ID #15618355.

Mouse tail

Yes

Yes

Tail needs to be treated with proteinase K before purification. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section.
Reference:
Nielsen, J.V. et al. (2007) Development 134, 1133–40.
PubMed ID #17301088.

Mouse brain

Yes

Sinz, E.H. et al. (1999) J. Clin. Invest. 104, 647–56.
PubMed ID #10487779.

Mouse Lymphoma

Yes

Yu, Z. et al. (2006) Cancer Res. 66, 755–62.
PubMed ID #16424006.

Rat skeletal muscle, liver and heart

Yes

Barazzoni, R. et al. (2000) J. Biol. Chem. 275, 3343–7.
PubMed ID #10652323.

Human bone marrow aspirate

Yes

Yu, Z. et al. (2006) Cancer Res. 66, 755–62.
PubMed ID #16424006.

Human liver and testicle

Yes

Maduro, M.R. et al. (2003) Mol. Hum. Reprod. 9, 61–8.
PubMed ID #12569174.

Human colon

Yes

Tost, J. et al. (2003) Nucleic Acids Res. 31, e50.
PubMed ID #12711695.

Tomato leaf

Yes

Leaf needs to be frozen in liquid nitrogen and ground with a mortar and pestle prior to adding lysis buffer. A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Plant Tissue" section.

Arabidopsis

Yes

Vorwerk, S. (2001) Wizard® Genomic DNA Purification Kit and the isolation of plant genomic DNA. eNotes

Maize

Yes

Vorwerk, S. (2001) Wizard® Genomic DNA Purification Kit and the isolation of plant genomic DNA. eNotes

Lemna minor (aquatic plant)

Yes

Stout, L.M. and Nüsslein, K. (2005) Appl. Environ. Microbiol. 71, 2484–92.
PubMed ID #15870338.

Medicago truncatula

Yes

Total DNA was isolated from roots, which may include various mycorrhizal fungi.
Kosuta, S. et al. (2003) Plant Physiol. 131, 952–62.
PubMed ID #12644648.

Tetrahymena thermophila

Yes

Followed plant DNA protocol ("Isolating Genomic DNA from Plant Tissue"), but skipped freezing in liquid nitrogen and grinding with a mortar and pestle.
Jacobs, M.E. et al. (2006) Eukaryot. Cell 5, 1990–2000.
PubMed ID #17012537.

Drosophila D.Mel-2 embryonic cells

Yes

Roberti, M. et al. (2006) Nucleic Acids Res. 34, 2109–16.
PubMed ID #16648357.

Mouse embryonic stem cells

Yes

Oka, M. et al. (2006) J. Biol. Chem. 281, 9901–8.
PubMed ID #16439359.

Monkey primary fibroblasts, virus-infected

Yes

Cells were lysed using 300 µl of Nuclei Lysis Solution with proteinase K and incubating overnight at 37°C.
Mansfield, K.G. et al. (1999) J. Virol. 73, 10320–8.
PubMed ID #10559350.

Vero cells infected with HSV-1

Yes

Both viral and genomic DNA were isolated.
Bower, J.R. et al. (1999) J. Virol. 73, 3843–53.
PubMed ID #10196279.

CD45RA+ T cells from cord blood

Yes

Samples of 2–4 × 10⁶ cells were pelleted, washed with PBS, snap frozen in liquid nitrogen and frozen at –70°C prior to using the Wizard® Genomic DNA Purification Kit. Soares et al. (1998) J. Immunol. 161, 5909–17.
PubMed ID #9834071.

Enterobacter cloacae

Yes

A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria" section.

Escherichia coli

Yes

Yes

A protocol is provided in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, "Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria" section.
Reference:
Zheng, W. et al. (2001) EMBO J. 20, 1164–72.
PubMed ID #11230139.

Nitrobacter winogradskyi

Yes

Starkenburg, S.R. et al. (2006) Appl. Environ. Microbiol. 72, 2050–63.
PubMed ID #16517654.

Yersinia pestis

Yes

Flashner, Y. et al. (2004) Infect. Immun. 72, 908–15.
PubMed ID #14742535.

Burkholderia species

Yes

Moore, R.A. et al. (2004) Infect. Immun. 72, 4172–87.
PubMed ID #15213162.

Salmonella enterica

Yes

Nair, S. et al. (2004) J. Bacteriol. 186, 3214–23.
PubMed ID #15126484.

Field bacteria samples, non-Agrobacterium

Yes

Weller, S.A. et al. (2004) Appl. Environ. Microbiol. 70, 2779–85.
PubMed ID #15128532.

Haemophilus influenzae

Yes

Ottemann, K.M. and Lowenthal, A.C. (2002) Infect. Immun. 70, 1984–90.
PubMed ID #11895962.

Bordetella bronchiseptica

Yes

Brockmeier, S.L. et al. (2002) Infect. Immun. 70, 481–90.
PubMed ID #11796573.

Leptospira kirschneri

Yes

Matsunaga, J. et al. (2002) Infect. Immun. 70, 323–34.
PubMed ID #11748198.

Klebsiella pneumoniae

Yes

Yigit, H. et al. (2001) Antimicrob. Agents Chemother. 45, 1151–61.
PubMed ID #11257029.

Marine Geobacteraceae

Yes

Bacteria colonizing energy-harvesting anodes were scraped off, organically extracted and purified using the Wizard® Genomic DNA Purification Kit.
Bond, D.R. et al. (2002) Science 295, 483–5.
PubMed ID #11799240.

Serratia marcescens

Yes

Peccia, J. and Hernandez, M. (2001) Appl. Environ. Microbiol. 67, 4225–32.
PubMed ID #11526027.

Borrelia burgdorferi

Yes

Chaconas, G. et al. (2001) EMBO J. 20, 3229–37.
PubMed ID #11406599.

Campylobacter jejuni

Yes

Dorrell, N. et al.(2001) Genome Res. 11, 1706–15.
PubMed ID #11591647.

Desulfovibrio desulfuricans

Yes

Rapp-Giles, B.J. et al. (2000) Appl. Environ. Microbiol. 66, 671–7.
PubMed ID #10653734.

Vibrio cholerae

Yes

Stine, O.C. et al. (2000) Infect. Immun. 68, 7180–5.
PubMed ID #11083852.

Vibrio harveyi

Yes

Zhou, W. et al. (1999) Biochemistry 38, 16246–52.
PubMed ID #10587447.

Paracoccus denitrificans

Yes

Shearer, N. et al. (1999) J. Bacteriol. 181, 6907–13.
PubMed ID #10559155.

Campylobacter species

Yes

Before genomic DNA isolation, the bacteria were washed in TE buffer and resuspended in 1ml of TE buffer at ~1 × 10⁹ cells/ml.
Duim, B. et al. (1999) Appl. Environ. Microbiol. 65, 2369–75.
PubMed ID #10347015.

Sphingomonas paucimobilis

Yes

Jiang, S.C. et al. (1998) Appl. Environ. Microbiol. 64, 535–42.
PubMed ID #9464390.

Flavobacterium species

Yes

Jiang, S.C. et al. (1998) Appl. Environ. Microbiol. 64, 535–42.
PubMed ID #9464390.

Bacillus anthracis

Yes

Pomerantsev, A.P. et al. (2006) Infect. Immun. 74, 682–93.
PubMed ID #16369025.

Bacillus cereus

Yes

van Schaik, W. et al. (2004) J. Bacteriol. 186, 316–25.
PubMed ID #14702299.

Bacillus subtilis

Yes

Ben-Yehuda, S. and Losick, R. (2002) Cell 109, 257–66.
PubMed ID #12007411.

Staphylococcus, 12 strains

Yes

Li, Y. et al. (2003) J. Clin. Microbiol. 41, 3481–6.
PubMed ID #12904342.

Lactobacillus acidophilus

Yes

Li, Y. et al. (2003) J. Clin. Microbiol. 41, 3481–6.
PubMed ID #12904342.

Actinomyces naeslundii

Yes

Li, Y. et al. (2003) J. Clin. Microbiol. 41, 3481–6.
PubMed ID #12904342.

Streptococcus pneumoniae

Yes

Brown, J.S. et al. (2002) Infect. Immun. 70, 4389–98.
PubMed ID #12117949.

Methanococcus jannaschii

Yes

Löwe, J. and Amos, L.A. (1998) Nature 391, 203–6.
PubMed ID #9428770.

Methanosarcina mazei

Yes

Lange, M. et al. (2000) Appl. Environ. Microbiol. 66, 1796–1800.
PubMed ID #10788341.

Mycoplasma hyorhinis

Yes

Cells were collected and lysed in 1% sodium dodecyl sulfate (SDS), 45mM Tris (pH 8.0) and 9mM EDTA for 10 minutes at 50°C, then incubated for 10 minutes at 37°C. The Wizard® Genomic DNA Purification Kit was subsequently used for genomic DNA extraction.
Citti, C. et al. (2000) J. Bacteriol. 182, 1356–63.
PubMed ID #10671459.

Mycobacterium parafortuitum

Yes

Cells were treated with proteinase K and lysozyme for
1 hour, centrifuged and resuspended in Nuclei Lysis Solution. Glass beads were added, the glass bead-cell lysate shaken for 5 minutes, incubated at 65°C for 1 hour and the glass bead-lysate shaken again for 3 minutes. The protocol then followed the bacterial method of the Wizard® Genomic DNA Purification Kit ("Isolating Genomic DNA from Gram Positive and Gram Negative Bacteria").
Peccia, J. and Hernandez, M. (2001) Appl. Environ. Microbiol. 67, 4225–32.
PubMed ID #11526027.

Intestinal microflora

Yes

Chicken intestines were removed and the contents of each segment (duodenum, jejunum, ileum, and cecum) were inverted into a sterile 15ml tube containing 9ml of sterile PBS. After adding 4mm glass beads, the samples were vortexed for 3 minutes, and the debris was removed by centrifuging at 700 × g for 1 minute. The supernatant was collected and centrifuged at 12,000 × g for 5 minutes. The pellet was washed twice with PBS and stored at –20°C until DNA extraction. This frozen pellet was resuspended in EDTA and treated with lysozyme (at a final concentration of 10mg/ml) for 45 minutes at 37°C. The bacterial genomic DNA was isolated with the Wizard® Genomic DNA Purification Kit.
Amit-Romach, E., Sklan, D. and Uni, Z. (2004) Poult. Sci. 83, 1093–98.
PubMed ID #15285498

Candida species, various

Yes

Yeast cells were incubated for 1 hour at 37°C with 1 M sorbitol, 0.1 M EDTA, 0.1% (w/v) zymolyase-100T (Seikaguku Corp.), and 1% (v/v) 2-mercaptoethanol adjusted to pH 7.5. Chromosomal DNA was then isolated using the Wizard® Genomic DNA Purification Kit.
Park, S. et al.(2000) J. Clin. Microbiol. 38, 2829–36.
PubMed ID #10921935.

Aspergillus fumigatus

Yes

Cells were filtered through a 0.2µm SFCA filter, frozen on dry ice, then ground with a mortar and pestle. The ground cells were incubated in Nuclei Lysis Solution at 37°C for 1 hour before purifying with the Wizard® Genomic DNA Purification Kit," Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section.
Burghoorn, H.P. et al. (2002) Antimicrob. Agents Chemother. 46, 615–24.
PubMed ID #11850239.

Nectria haematococca

Yes

Mycelia were ground in liquid nitrogen and lyophilized before lysing in a modified lysis buffer consisting of 50 mM Tris-HCl (pH 7.5), 50 mM EDTA (pH 8.0), 3% SDS and 1% mercaptoethanol.
Wu, Q. et al. (1998) Mol. Biol. Cell 9, 89–101.
PubMed ID #9436993.

Pneumocystis carinii

Yes

Sputum samples were dispensed into ~1ml aliquots and centrifuged at 14,000 × g for 5–7 minutes. The cell pellet was resuspended in 1 ml of phosphate-buffered saline (0.01M, pH 7.2) containing 1mM EDTA (PBS-EDTA), washed twice in PBS-EDTA, centrifuged and stored at –80°C for later DNA extraction. DNA was prepared using the Wizard® Genomic DNA Purification Kit, "Isolating Genomic DNA from Whole Blood " section.
Beard, C.B. et al. (2000) Emerg. Infect. Dis. 6, 265–72.
PubMed ID #10827116.

Drosophila, adult fly

Yes

To prepare genomic DNA from adult D. virilis and D. melanogaster, 100 adult flies were homogenized and the DNA purified using the Wizard® Genomic DNA Purification Kit.
Hanrahan, C.J. et al. (2000) Genetics 155, 1149–60.
PubMed ID #10880477.

Frog Skin

Yes

Skin biopsies from Amphibiocystidium ranae (10µm sections) were deparaffinized twice in xylene and centrifuged at high speed. The tissue pellet was washed with 95% and 70% ethanol, then dried, and the genomic DNA was extracted using the Wizard® Genomic DNA Purification Kit.
Pereira, C.N. et al. (2005) J. Clin. Microbiol. 43, 192–8.
PubMed ID #15634971.

Cichlid fin and whole fish

Yes

Genomic DNA was isolated from 1–2 mm2 ethanol-preserved Neolamprologus pulcher finclip samples or whole offspring using the Wizard® Genomic DNA Isolation Kit, "Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section, with small modifications (not listed).
Heg, D. et al. (2006) Behav. Ecol. 17, 419–29.

Plasmodium vivax

Yes

Researchers used a modified animal tissue/tail snip protocol.
Sawabe, T and Ikeda, T. (2005) Wizard® Genomic DNA Purification Kit provides high-quality genomic DNA template for molecular phylogenetic studies on Copepod crustaceans. eNotes

Plasmodium vivax

Yes

To isolate the parasite from whole blood, a modified protocol was used.

1. Centrifuge 15ml vials containing infected sodium-citrate whole blood at 3,000rpm for 10 minutes.
2. Extract serum and leukocytes with 5ml pipettes.
3. Dilute erythrocytes in 7 volumes of 0.15% saponin.
4. Incubate at 37°C for 1 hour.
5. Centrifuge at 3,000rpm for 40 minutes.
6. Discard supernatant, taking care not to disturb the parasite pellet.
7. Resuspend the pellet in 15ml of 1X PBS and centrifuge at 3,000rpm for 10 minutes. Discard the supernatant, and repeat the procedure twice.

Start with the Nuclei Lysis Solution addition step in the "Isolating Genomic DNA from Whole Blood" section of the Wizard® Genomic DNA Purification Kit Technical Manual #TM050.
(Personal communication)

Amoeba

Yes

Trophozoites of clone A of Entamoeba histolytica (strain HM1:IMSS) were "axenically cultured" in TYI-S33 medium. Trophozoites (3 × 10⁶) were processed as described in the Wizard® Genomic DNA Purification Kit Technical Manual #TM050, following the protocol for isolation of genomic DNA from tissue culture cells ("Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue" section). DNA pellet was dissolved with 50µl of DNA rehydration solution and incubated overnight at 4°C. Yield: 2.5µg per 3 × 10⁶ trophozoites. Purity A_260/280: 1.8.
(Personal communication)