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Immobilized Trypsin

Fast, Convenient Digestion of Purified Protein or Protein Mixtures

  • Digest a range of protein concentrations
  • Digestions completed in as little as 30 minutes

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Catalog number selected: V9012

$ 256.00
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Immobilized Trypsin
2ml
$ 256.00
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Immobilized Trypsin provides a fast and convenient method for digesting a range of concentrations of purified protein or complex protein mixtures. Digested peptides are easily separated from the Immobilized Trypsin as they flow through the spin column into the collection tube. Immobilized Trypsin is easily removed from the peptide solution because the trypsin does not pass though the column frit. Trypsin is a proteolytic enzyme, which cleaves at the carboxyl side of positively charged Lysine (Lys) and Arginine (Arg). When these amino acids are followed by the nonpolar Proline (Pro), the digestion of the site is not efficient. When Lys and Arg are followed by acids [Aspartic Acid (Asp) and Glutamic Acid (Glu)] the digestion is also not as efficient.

Immobilized Trypsin lets you perform digestions in as little as 30 minutes. The easily adjustable protocol easily accommodates various protein concentrations. No shaking or water baths needed.

Applications

  • Protein identification
  • Quantitative analysis
  • Differential expression
  • Post-translational modifications

Overview of the Immobilized Trypsin digestion protocol.

Overview of the Immobilized Trypsin digestion protocol.

Proteins are denatured, reduced and alkylated as appropriate before digestion. The protein digestion solution containing the protein to be digested is prepared and added to the Immobilized Trypsin resin. The protein digestion solution is incubated with the resin for 30 minutes. Digested peptides are removed by centrifugation and then prepared for downstream analysis.

Specifications

You are viewing: V9012 Change Configuration

What's in the box?

Item Part # Size

Spin Columns

V138A 2 × 5 each

Immobilized Trypsin

V901B 1 × 2ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

Specifications

You are viewing: V9013 Change Configuration

What's in the box?

Item Part # Size

Spin Columns

V138A 4 × 5 each

Immobilized Trypsin

V901B 2 × 2ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

CC

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