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Trypsin Gold, Mass Spectrometry Grade

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Maximum Trypsin Digest Specificity, Extreme Resistance to Autolytic Digestion

  • Each lot qualified by mass spectrometry to ensures compatibility with your applications/instrumentation
  • Chymotrypsin activity eliminated for distinct, consistent data
  • Digest in gel, or in solution


Catalog number selected: V5280

$ 110.00
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Trypsin Gold, Mass Spectrometry Grade
$ 110.00
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Trypsin Digest overview

Excellent Specificity and Resistance to Autolytic Digestion

Trypsin Gold, Mass Spectrometry Grade, is manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin. The treated trypsin is then purified by affinity chromatography and lyophilized to yield Trypsin Gold, Mass Spectrometry Grade.

Each lot of quality-tested Trypsin Gold, Mass Spectrometry Grade, is qualified for use with in-gel protein digestion and mass spectrometric analysis. For more information, see the Protein Purification and Analysis chapter of the Protocols and Applications Guide.

See our newest trypsin: Rapid Digestion-Trypsin and Rapid Digestion-Trypsin/Lys-C

Learn more about our custom options for this product at: www.promega.com/custom/

Spectrogram Showing Digestion with Trypsin Gold, Mass Spec Grade


A 500ng sample of carbonic anhydrase II was separated by gel electrophoresis and digested with 500ng of Trypsin Gold, Mass Spectrometry Grade, overnight at 37°C.


What's in the box?

Item Part # Size

Trypsin Gold, Mass Spectrometry Grade

V528A 1 × 100μg


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Download SDSPDF (49 KB) – English (United States)

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions


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